Study on the protective effect and mechanism of Dicliptera chinensis (L.) Juss (Acanthaceae) polysaccharide on immune liver injury induced by LPS
Study on the protective effect and mechanism of Dicliptera chinensis (L.) Juss (Acanthaceae) polysaccharide on immune liver injury induced by LPS
复制标题
棘科双翅目多糖对脂多糖所致免疫性肝损伤的保护作用及机制研究
DOI：
10.1016/j.biopha.2020.111159
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发表时间：
2021
影响因子：
7.5
通讯作者：
Gao Ya
中科院分区：
文献类型：
--
作者：
Xu Qiongmei;Xu Jie;Zhang Kefeng;Zhong Mingli;Cao Houkang;Wei Riming;Jin Ling;Gao Ya
本研究旨在利用狗肝菜Dicliptera chinensis（L.）目的：探讨酸枣仁多糖（DCP）对NF-κB B炎症通路和Fas/FasL配体系统的影响，以期寻找改善免疫性肝损伤的新途径。采用脂多糖（LPS）建立昆明小鼠体内和体外损伤模型（LO 2细胞）。本实验采用苏木精-伊红（H&E）染色及相关生化指标，观察肝组织病理变化、氧化应激及炎症反应。采用免疫组化、ELISA、RT-PCR和Western blot检测炎症反应和凋亡相关蛋白或mRNA的表达。实验结果表明，模型组肝细胞损伤明显，有炎症浸润。DCP干预后能显著降低血清ALT、AST、ALP、TBIL和MDA水平，提高SOD和GSH-Px含量。此外，DCP可降低肝脏NF-κB的表达水平，减少下游炎症因子TNF-α、IL-6和IL-1β的释放，从而减轻炎症。同时，DCP可显著抑制Fas/FasL配体系统及凋亡相关蛋白和mRNA的表达，从而减少细胞凋亡。结论DCP可通过抑制肝脏炎症和细胞凋亡减轻肝损伤，为临床治疗免疫性肝损伤提供了新的策略。
The purpose of this study is to useDicliptera chinensis(L.) Juss (Acanthaceae) polysaccharide (DCP) to act on the NF-κB inflammatory pathway and Fas/FasL ligand system, in order to find a new method to improve immune liver injury. Lipopolysaccharide (LPS) was used to establish an injury modelin vivo(Kunming mice) andin vitro(LO2 cells). In this experiment, hematoxylin-eosin (H&E) staining and related biochemical indicators were used to observe the pathological changes of liver tissues, oxidative stress and inflammatory reactions. Immunohistochemistry, ELISA, RT-PCR and Western blot were used to detect protein or mRNA expressions associated with inflammation response and apoptosis. The experimental results show that the model group has obvious liver cell damage and inflammatory infiltration. After DCP intervention, it could significantly reduce the levels of ALT, AST, ALP, TBIL and MDA in serum, and increase the content of SOD and GSH-Px. In addition, DCP can reduce the expression level of NF-κB in the liver and reduce the release of downstream inflammatory factors TNF-α, IL-6 and IL-1β, thereby reducing the inflammation. At the same time, DCP can significantly inhibit the expression of Fas/FasL ligand system and apoptosis related-proteins and mRNA, which in turn can reduce cell apoptosis. In conclusion, DCP can alleviate liver injury by inhibiting liver inflammation and apoptosis, which provides a new strategy for clinical treatment of immune liver injury.