Effects of Excess Copper Ions on Decidualization of Human Endometrial Stromal Cells
Effects of Excess Copper Ions on Decidualization of Human Endometrial Stromal Cells
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过量铜离子对人子宫内膜基质细胞蜕膜化的影响
DOI：
10.1007/s12011-016-0840-y
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发表时间：
2017-05-01
影响因子：
3.9
通讯作者：
Yang, Zeng-Ming
中科院分区：
文献类型：
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作者：
Li, Ying;Kang, Zhen-Long;Yang, Zeng-Ming
本研究旨在探讨铜离子对体外培养的人子宫内膜基质细胞（HESCs）脱体细胞化的影响。首先，根据细胞活性筛选HESCs中d -葡萄糖酸铜的无毒浓度。然后，研究无毒浓度（0~250 μM）铜离子对人子宫内膜间质细胞去个体化的影响。我们的数据表明，在100 μM或250 μM铜离子处理下，胰岛素样生长因子结合蛋白（IGFBP-1）、泌乳素（PRL）、Mn-SOD和foxo1的mRNA表达在去个性化过程中下调。同时，HESCs上清液中丙二醛（MDA）含量增加。这些结果表明，铜处理对HESCs的体外脱体细胞化有损害。
The aim of this study was to investigate the effects of copper ions on decidualization of human endometrial stromal cells (HESCs) cultured in vitro. Firstly, non-toxic concentrations of copper D-gluconate were screened in HESCs based on cell activity. Then, the effects of non-toxic concentrations of copper ions (0~250 μM) were examined on decidualization of human endometrial stromal cells. Our data demonstrated that the mRNA expressions of insulin-like growth factor binding protein (IGFBP-1), prolactin (PRL), Mn-SOD, and FOXO1were down-regulated during decidualization following the treatments with 100 or 250 μM copper ions. Meanwhile, the amount of malonaldehyde (MDA) in the supernatant of HESCs was increased. These results showed that in vitro decidualization of HESCs was impaired by copper treatment.