Investigation of neutron-induced damage in DNA by atomic force microscopy: Experimental evidence of clustered DNA lesions
Investigation of neutron-induced damage in DNA by atomic force microscopy: Experimental evidence of clustered DNA lesions
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DOI：
10.2307/3579883
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发表时间：
1998-12-01
影响因子：
3.4
通讯作者：
Dritschilo, A
中科院分区：
文献类型：
--
作者：
Pang, D;Berman, BL;Dritschilo, A
利用原子力显微镜（AFM）研究了水溶液中中子诱导的质粒DNA双链断裂。原子力显微镜允许以几纳米的精度直接测量单个DNA分子。此外，DNA片段大小分布的分析是非参数的，而其他方法依赖于模型。中子辐照DNA导致许多短片段的产生，这是对低let辐射引起的损伤所没有观察到的。这些数据为集群DNA双链断裂的存在提供了明确的实验证据，并证明在没有核小体DNA结构的情况下，这种辐射可能产生短的DNA片段。(C)辐射研究学会1998年出版。
Using atomic force microscopy (AFM), we have investigated neutron-induced DNA double-strand breaks in plasmids in aqueous solution. AFM permits direct measurement of individual DNA molecules with an accuracy of a few nanometers. Furthermore, the analysis of the DNA fragment size distribution is nonparametric, whereas other methods are dependent on the model. Neutron irradiation of DNA results in the generation of many short fragments, an observation not made for damage induced by low-LET radiation. These data provide clear experimental evidence for the existence of clustered DNA double-strand breaks and demonstrate that short DNA fragments may be produced by such radiations in the absence of a nucleosomal DNA structure. (C) 1998 by Radiation Research Society.