COLLAGEN FIBRILLOGENESIS IN-SITU - FIBRIL SEGMENTS UNDERGO POSTDEPOSITIONAL MODIFICATIONS RESULTING IN LINEAR AND LATERAL GROWTH DURING MATRIX DEVELOPMENT
COLLAGEN FIBRILLOGENESIS IN-SITU - FIBRIL SEGMENTS UNDERGO POSTDEPOSITIONAL MODIFICATIONS RESULTING IN LINEAR AND LATERAL GROWTH DURING MATRIX DEVELOPMENT
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DOI：
10.1002/aja.1002020303
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发表时间：
1995-03-01
影响因子：
2.5
通讯作者：
ZYCBAND, EI
中科院分区：
文献类型：
--
作者：
BIRK, DE;NURMINSKAYA, MV;ZYCBAND, EI
阐明胶原原纤维的生长是如何被调节的，对于确定组织是如何组装的很重要。纤维以片段的形式沉积，这些片段的生长是组织结构、稳定性和机械属性的重要决定因素。从发育中的肌腱中分离出纤维段，并对其结构进行了表征，还观察了沉积后导致纤维线状和侧向生长的变化。从14日龄鸡胚腱中提取的纤维段的平均长度为29微米，段不对称，末端短而长的锥形。大多数链段在分子堆积方面是中心对称的。从12至16天的肌腱提取的节段具有相同的结构，但由于越来越多的较长节段的增加，平均节段长度逐渐增加。在发育到第17天时，节段长度急剧增加，形态数据表明，长度的增加是相邻节段之间横向关联的结果，分析表明，这种原纤维的生长与原纤维相关的核心蛋白显著减少有关。用免疫电子显微镜观察，在发育第18天，核心蛋白聚糖显著减少。当用生化方法测定核心蛋白的含量时，也观察到了减少。在同一时期，核心蛋白聚糖的mRNA相对纤维状胶原的mRNA也降低了。这些数据支持这样的假设，即随着组织的成熟，纤维相关的核心蛋白聚糖的减少对于纤维的生长是必要的。通过沉积后融合的生长允许同位生长和间歇生长，对于正常发育、生长和修复是必不可少的。(C)1995年Wiley-Liss公司
Elucidating how collagen fibril growth is regulated is important in determining how tissues are assembled. Fibrils are deposited as segments, The growth of these segments is an important determinant of tissue architecture, stability, and mechanical attributes. Fibril segments were isolated from developing tendons and their structure characterized, The post-depositional changes leading to Linear and lateral growth of fibrils also were examined, Segments extracted from 14-day chicken embryo tendons had a mean length of 29 mu m. The segments were asymmetric, having a short and a long tapered end. Most of the segments were centrosymmetric with respect to molecular packing. Segments extracted from 12-to 16-day tendons had the same structure, but mean segment length increased incrementally due to the addition of an increasingly large population of longer segments. At 17 days of development there was a precipitous increase in segment length, The morphological data indicate that the increase in length was the result of lateral associations among adjacent segments, Analysis demonstrated that this fibril growth was associated with a significant decrease in fibril associated decorin. Using immunoelectron microscopy, decorin was seen to decrease significantly at 18 days of development. When decorin content was biochemically determined, a decrease also was observed. Decorin mRNA also decreased relative to fibrillar collagen mRNA during the same period. These data support the hypothesis that a decrease in fibril-associated decorin is necessary for fibril growth associated with tissue maturation. Growth through post-depositional fusion allows for appositional and intercalary growth and would be essential for normal development, growth, and repair. (C) 1995 Wiley-Liss, Inc.