Overexpression of OsJAC1, a lectin gene, suppresses the coleoptile and stem elongation in rice
Overexpression of OsJAC1, a lectin gene, suppresses the coleoptile and stem elongation in rice
复制标题
DOI：
10.1111/j.1744-7909.2007.00428.x
复制
发表时间：
2007-02-01
影响因子：
11.4
通讯作者：
Chong, Kang
中科院分区：
文献类型：
--
作者：
Jiang, Jia-Fu;Xu, Yun-Yuan;Chong, Kang
凝集素在植物防御信号传导中发挥重要作用，但其在植物生长和发育中的功能尚不清楚。此前，我们克隆了水稻（Oryza sativa L.）编码甘露糖结合jacalin相关凝集素的基因OsJAC1，并发现OsJAC1是茉莉酸（JA）诱导型的。我们克隆了OsJAC1的启动子，在OsJAC1::GUS转基因水稻的幼根、胚芽鞘、鞘、叶、茎节、茎、轴、雌蕊、雄蕊和外稃中检测到了GUS活性，表明OsJAC1是水稻中的组成型表达基因。此外，OsJAC1 过表达 (Ubi::OsJAC1) 水稻由于胚芽鞘细胞伸长失败而表现出矮化，胚芽鞘较短。此外，与野生型植物的胚芽鞘相比，OsJAC1过表达水稻的胚芽鞘对JA处理更敏感。这些数据表明，除了在防御反应中发挥作用外，凝集素在水稻生长和发育中也发挥着重要作用。
Lectin plays an important role in defense signaling in plants, but its function in plant growth and development is not well known. Previously, we cloned a rice (Oryza sativa L.) gene OsJAC1 encoding a mannose-binding jacalin-related lectin, and found that OsJAC1 was jasmonic acid (JA) inducible. Here we cloned the promoter of OsJAC1, and GUS activity was detected in young roots, coleoptiles, sheaths, leaves, nodes of stems, stems, rachises, pistils, stamens and lemmas of OsJAC1::GUS transgenic rice, suggesting that OsJAC1 is a constitutive expression gene in rice. Moreover, OsJAC1-overexpressed (Ubi::OsJAC1) rice showed dwarfism with shorter coleptiles resulting from the failure of cell elongation of coleoptiles. In addition, compared with coleoptiles of wild-type plants, those of OsJAC1 overexpression rice were more sensitive to JA treatment. These data revealed that, besides its roles in defense response, lectin plays an important role in rice growth and development.