ESTROGEN INDUCES INSULIN-LIKE GROWTH FACTOR-I EXPRESSION IN THE RAT UTERUS
ESTROGEN INDUCES INSULIN-LIKE GROWTH FACTOR-I EXPRESSION IN THE RAT UTERUS
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DOI：
10.1210/mend-1-7-445
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发表时间：
1987-07-01
影响因子：
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通讯作者：
FRIESEN, HG
中科院分区：
文献类型：
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作者：
MURPHY, LJ;MURPHY, LC;FRIESEN, HG
不能令人信服地证明雌激素对离体培养的子宫细胞的促有丝分裂作用表明，自分泌或旁分泌生长因子可能是重要的雌激素诱导的子宫增殖反应。在此，我们报道了GH作用的重要介质胰岛素样生长因子-I（IGF-I）的子宫表达在17 β-雌二醇后增加。雌二醇（5 μ g/100 g bw，ip）给药至卵巢切除的青春期前大鼠。子宫IGF-I mRNA丰度的增加，约14倍以上未经处理的对照组，是明显的雌激素给药后6小时，达到的水平超过了完整的成熟大鼠在动情间期子宫中看到的。与子宫中IGF-I表达的增加相反，在17 β-雌二醇后，血清IGF-I浓度或肝或肾IGF-I mRNA丰度没有显著变化。雌二醇注射的卵巢切除青春期前大鼠。子宫IGF-I表达的增加在垂体完整和垂体切除、卵巢切除大鼠中相似。我们相信这是第一个报告的诱导IGF-Ⅰ的表达，雌激素在体内。因此，这一发现扩大了IGF-I作为生长介质的作用和意义，超越了与GH相关的作用和意义。
The inability to convincingly demonstrate a mitogenic effect of estrogen on isolated uterine cells in culture suggests that autocrine or paracrine growth factors may be important in the estrogen-induced uterine proliferative response. Here we report that uterine expression of insulin-like growth factor-I (IGF-I), an important mediator of GH action, is increased after 17.beta.-estradiol (5 .mu.g/100 g bw, ip) administration to ovariectomized prepubertal rats. An increase in uterine IGF-I mRNA abundance, approximately 14-fold above untreated controls, was apparent 6 h after estrogen administration and the level achieved exceeded that seen in the uterus from intact mature rats during diestrus. In contrast to the increase in IGF-I expression in the uterus, no significant change in serum IGF-I concentration or hepatic or renal IGF-I mRNA abundance was demonstrable after 17.beta.-estradiol injection of ovariectomized prepubertal rats. The increase in uterine IGF-I expression, was similar in both pituitary-intact and hypophysectomized, ovariectomized rats. We believe this is the first report of induction of IGF-I expression by estrogen in vivo. As such, the finding expands the role and significance of IGF-I as a mediator of growth beyond that related to GH.