Distribution and contribution of calcium from the albumen, yolk and shell to the developing chick embryo.
Distribution and contribution of calcium from the albumen, yolk and shell to the developing chick embryo.
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蛋白、蛋黄和蛋壳中钙的分布和对发育中鸡胚的贡献。
DOI：
10.1152/ajplegacy.1955.183.3.365
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发表时间：
1955
期刊：
影响因子：
--
通讯作者：
C. Comar
中科院分区：
文献类型：
--
作者：
P. M. Johnston;C. Comar
方法从当地孵化场获得混合品种的受精卵，主要是奥平顿。在温度平衡后，称量每个蛋的重量，并注射0.2 ml含有25 μ g Ca 45作为氯化物的溶液;比活度为12.97 mg钙。所用鸡蛋的平均重量为61.8和3.5克。使用O型注射器通过鸡蛋的小端注射到致密的蛋白中。5 ml结核菌素注射器，安装在Krogh-Keys移液器中，配有$-英寸27号针头。在
METHODSFertile eggs of mixed breeds, mostly Orpington, were obtained from a local hatchery. After allowing for temperature equilibration, each egg was weighed and injected with 0.2 ml of solution containing 25 pc of Ca 45 as the chloride; the specific activity was 12.97 &mg of calcium. The mean weight for the eggs used was 61.8 & 3.5 grams. Injection was made through the small end of the egg into the dense albumen using a o. 5-ml tuberculin syringe, mounted in a Krogh-Keys pipetter, equipped with a $-inch 27 gauge needle. In