Complete genome sequence of a Chuzan virus strain isolated for the first time in mainland China
Complete genome sequence of a Chuzan virus strain isolated for the first time in mainland China
复制标题
中国大陆首次分离的中山病毒株全基因组序列。
DOI：
10.1007/s00705-015-2734-2
复制
发表时间：
2016-04-01
影响因子：
2.7
通讯作者：
Li, Huachun
中科院分区：
文献类型：
--
作者：
Yang, Heng;Xiao, Lei;Li, Huachun
Chuzan病毒（CHUV）属于Palyam血清群，引起牛先天性疾病，在亚洲流行。迄今为止，仅报道了一种完整的帕亚姆病毒 (PALV) 基因组序列，即日本 CHUV 毒株 K47 的序列。序列分析表明，不同地理区域分离的PALV毒株表现出显着的多样性，这主要是由地理独立进化和遗传重排形成的。我们对 PALV 遗传特征的理解受到非常有限的基因组序列数据库的阻碍。在本研究中，我们报告了CHUV毒株SZ187的完整基因组序列，该毒株于2012年在中国大陆首次分离。序列比对和系统发育分析表明，SZ187 与台湾和日本分离的其他 CHUV 毒株密切相关，表明它们可能拥有共同的祖先。这种新的全长 CHUV 基因组序列可以帮助设计更广泛的流行病学研究检测方法，并有助于将来识别新的 CHUV 分离株。
Chuzan virus (CHUV) belongs to the Palyam serogroup, causes bovine congenital disease, and is prevalent in Asia. To date, only one full Palyam virus (PALV) genome sequence, that of Japanese CHUV strain K47, has been reported. Sequence analysis indicates that PALV strains isolated from different geographical regions show significant diversity, which is mainly shaped by geographically independent evolution and genetic reassortment. Our understanding of the genetic characteristics of PALV is hampered by a very limited genomic sequence database. In this study, we report the complete genome sequence of CHUV strain SZ187, which was isolated for the first time in 2012 in mainland China. Sequence alignment and phylogenetic analysis demonstrate that SZ187 is closely related to other CHUV strains isolated in Taiwan and Japan, indicating that they may share a common ancestor. This new full-length CHUV genome sequence could help in the design of broader assays for epidemiological studies and facilitate the identification of new CHUV isolates in the future.