Longitudinal Changes in the Composition of the Penile Microbiome Are Associated With Circumcision Status, HIV and HSV-2 Status, Sexual Practices, and Female Partner Microbiome Composition.
Longitudinal Changes in the Composition of the Penile Microbiome Are Associated With Circumcision Status, HIV and HSV-2 Status, Sexual Practices, and Female Partner Microbiome Composition.
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DOI：
10.3389/fcimb.2022.916437
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发表时间：
2022
影响因子：
5.7
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中科院分区：
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阴茎微生物组组成与男性中的HSV-2和HIV以及女性性伴侣中的细菌性阴道病（BV）和HSV-2相关。本研究旨在1）表征1年内的阴茎微生物组组成，2）确定随时间推移与阴茎微生物组组成相关的因素。这项对肯尼亚社区招募的异性恋夫妇进行的前瞻性研究通过16 S核糖体RNA基因扩增子测序在1年内的4个时间点（基线后1，6和12个月）测量了阴茎和阴道微生物组。我们使用纵向混合效应模型来评估相关的人口统计学，行为和疾病因素以及社区类型的变化，具有最高平均相对丰度的MEATAL类群，以及α和β多样性指标。我们估计组为基础的轨迹，以阐明成分的趋势。在218名男性中，有740名观察者，男性的中位年龄为26岁，11.6%的人感染艾滋病毒，46.1%的人HSV-2血清阳性。我们确定了7种阴茎群落类型，这些类型因包皮环切术状态、女性伴侣阴道微生物群落状态类型（CST）、安全套使用和阴茎清洗而异。在不同的分析方法中，50%-60%的男性具有稳定的阴茎微生物组组成。α多样性措施较低的包皮环切术的男子和那些谁报告使用避孕套，他们是稳定的，随着时间的推移，但如果女性伴侣有不同的CST或BV较高。BV与许多个体阴茎分类群的相对丰度呈正相关。Bray-Curtis相似性的降低在HSV-2男性中更为常见，HSV-2也与棒状杆菌和葡萄球菌的相对丰度较低相关。在1年的时间内，相当大比例的男性的阴茎微生物组组成是稳定的，并且受到男性包皮环切术状态、性行为、女性伴侣的阴道CST和BV状态以及男性的HSV-2状态的影响。在女性生殖道中，不同的CST通常与较差的健康结果有关。我们的研究结果有助于了解该框架是否扩展到阴茎微生物组，以及多样性和相关的阴茎微生物组组成是否影响男性对较差健康结果的易感性或恢复力。专注于了解这些因素如何影响阴茎微生物组可能会导致减少男性及其女性性伴侣中HSV-2和BV感染的治疗途径。
Penile microbiome composition has been associated with HSV-2 and HIV in men and with bacterial vaginosis (BV) and HSV-2 in female sex partners. This study sought to 1) characterize penile microbiome composition over a 1-year period and 2) identify factors associated with penile microbiome composition over time. This prospective study of community-recruited heterosexual couples in Kenya measured penile and vaginal microbiomes via 16S ribosomal RNA gene amplicon sequencing at 4 time points over 1 year (1, 6, and 12 months after baseline). We used longitudinal mixed-effects modeling to assess associated demographic, behavioral, and disease factors and changes in community type, meatal taxa with the highest mean relative abundance, and alpha and beta diversity measures. We estimated group-based trajectories to elucidate compositional trends. Among 218 men with 740 observations, men had a median age of 26 years, 11.6% were living with HIV, and 46.1% were HSV-2 seropositive. We identified 7 penile community types that varied with circumcision status, female partner vaginal microbiome community state type (CST), condom use, and penile washing. Across varying analytic approaches, 50%–60% of men had stable penile microbiome compositions. Alpha diversity measures were lower for circumcised men and those who reported condom use; they were stable over time but higher if female partners had diverse CSTs or BV. BV was positively associated with the relative abundance of numerous individual penile taxa. The decreased Bray–Curtis similarity was more common for men with HSV-2, and HSV-2 was also associated with a lower relative abundance of Corynebacterium and Staphylococcus. Over a 1-year period, penile microbiome composition was stable for a substantial proportion of men and was influenced by men’s circumcision status, sexual practices, female partner’s vaginal CST and BV status, and men’s HSV-2 status. In the female genital tract, a diverse CST is often associated with poorer health outcomes. Our results contribute toward understanding whether this framework extends to the penile microbiome and whether diversity and the associated penile microbiome compositions influence susceptibility or resilience to poorer health outcomes in men. Focusing on understanding how these factors influence the penile microbiome may lead to therapeutic avenues for reduced HSV-2 and BV infections in men and their female sex partners.