Microbial community structures in mixed bacterial consortia for azo dye treatment under aerobic and anaerobic conditions
Microbial community structures in mixed bacterial consortia for azo dye treatment under aerobic and anaerobic conditions
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需氧和厌氧条件下偶氮染料处理混合细菌群落的微生物群落结构
DOI：
10.1016/j.jhazmat.2012.04.032
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发表时间：
2012-06-30
影响因子：
13.6
通讯作者：
Zhao, Min
中科院分区：
文献类型：
--
作者：
Cui, Daizong;Li, Guofang;Zhao, Min
从染料废水中分离得到13株具有较高甲基红脱色能力的纯菌株。通过16 S rDNA测序鉴定每个分离物。结果表明，所分离的菌株均为兼性厌氧菌。开发了两种新的细菌复合体（AE和AN），分别在好氧和厌氧条件下对MR进行脱色。偶氮染料脱色率显着较高，使用财团相比，使用个别菌株。两株菌均能在短时间内对不同偶氮染料进行有效脱色，并能耐受高浓度的MR。为了进一步了解在好氧和厌氧条件下的细菌聚生体的微生物多样性，进行聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析。PCR-DGGE图谱显示，微生物群落发生了显着变化，不同的初始浓度的MR。系统发育分析表明，在好氧室的微生物种群属于克雷伯氏菌，布丘氏菌和芽孢杆菌，而克雷伯氏菌，大肠杆菌，芽孢杆菌和梭菌存在于厌氧室。克雷伯氏菌在两个菌群中均占优势，可能在偶氮染料的去除中起重要作用。(C)2012爱思唯尔有限公司版权所有。
Thirteen pure strains that possessed high methyl red (MR)-decolorizing ability were isolated from dye-contaminated water. Each isolate was identified by 16S rDNA sequencing. The results reveal that all of the isolated strains were facultative anaerobic bacteria. Two novel bacterial consortia (AE and AN), which could decolorize MR under aerobic and anaerobic conditions, respectively, were developed. Azo dye decolorization rate was significantly higher with the use of consortia compared to that with the use of individual strains. Both of the consortia can decolorize different azo dyes effectively in a short time, and tolerate MR with high concentrations. To provide further insight into the microbial diversity of the bacteria consortia under aerobic and anaerobic conditions, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses were performed. PCR-DGGE profiles revealed that the microbial community had changed significantly with varying initial concentrations of MR. Phylogenetic analysis indicated that microbial populations in the aerobic compartment belong to Klebsiella, Buttiauxella and Bacillus, whereas Klebsiella, Escherichia, Bacillus and Clostridium were present in the anaerobic compartment. Klebsiella, which was the majority genus in both of the consortia, may play an important role in azo dye removal. (C) 2012 Elsevier B.V. All rights reserved.