Gene Expression Profile of Dental Pulp Cells During Differentiation Into an Adipocyte Lineage
Gene Expression Profile of Dental Pulp Cells During Differentiation Into an Adipocyte Lineage
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DOI：
10.1254/jphs.10163fp
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发表时间：
2011-03-01
影响因子：
3.5
通讯作者：
Ohura, Kiyoshi
中科院分区：
文献类型：
--
作者：
Nozaki, Tadashige;Ohura, Kiyoshi
在牙髓来源的细胞中检测了体外分化为脂肪细胞的基因调控。加入胰岛素、3-异丁基-1-甲基黄嘌呤和地塞米松诱导脂肪生成。与3T3 L 1细胞一样，诱导后可观察到含有脂滴的细胞。大鼠牙髓来源细胞在体外具有向脂肪细胞分化的潜能。在两种类型的细胞中，多能标志物Oct-3/4和Sox2在分化过程中表达下调，而Nanog的表达在分化过程中没有明显变化。有趣的是，在牙髓来源的细胞中，Oct-3/4的水平在诱导后1周被瞬时诱导，然后在分化过程中显著下降。根据使用基因芯片阵列确定的表达谱，来自10个簇的3418个探针在诱导后1、2和3周与诱导前相比显示出不同的表达。值得注意的是，PPAR信号通路中的基因包括PPARγ、FABP4和C/EBP家族上调了3倍以上。在这种分化系统中，PPAR通路的上调似乎是一个关键的信号转导途径。这些发现表明牙髓来源的细胞是一种潜在的成脂细胞来源，其基因表达谱在未来的再生医学应用中可能是有用的。
Gene regulation during in vitro differentiation into adipocytes was examined in dental pulp derived cells. Insulin, 3-isobutyl-1-methylxanthine, and dexamethasone were added to induce adipogenesis. Cells containing lipid droplets were observed after induction as in 3T3 L 1 cells. Rat dental pulp derived cells showed their potential to differentiate into adipocytes in vitro. In both types of cells, the pluripotent markers Oct-3/4 and Sox2 were downregulated during differentiation, whereas the expression of Nanog was not significantly changed during differentiation. Interestingly, in the dental pulp derived cells, the level of Oct-3/4 was transiently induced at I week after induction and then significantly decreased during differentiation. Based on the expression profiles determined using GeneChip Arrays, 3418 probes across 10 clusters showed a difference in expression at 1, 2, and 3 weeks after induction versus before induction. Notably, genes in the PPAR signaling pathway including Ppar gamma, Fabp4, and the C/EBP family were upregulated by more than 3-fold. Upregulation of the PPAR pathways seems to be a critical signal transduction pathway in this differentiation system. These findings indicate that dental pulp derived cells are a potential source of adipogenic cells, and their gene expression profile could be useful in future regenerative medicine applications.