Polyphenols content and antioxidant capacity of thirteen faba bean (Vicia faba L.) genotypes cultivated in Tunisia
Polyphenols content and antioxidant capacity of thirteen faba bean (Vicia faba L.) genotypes cultivated in Tunisia
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DOI：
10.1016/j.foodres.2011.02.026
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发表时间：
2011-05-01
影响因子：
8.1
通讯作者：
Valiente, Manuel
中科院分区：
文献类型：
--
作者：
Chaieb, Nadia;Luis Gonzalez, Johannes;Valiente, Manuel
对13个蚕豆基因型的体外抗氧化能力进行了研究。在三个不同的生长阶段（营养，生殖和成熟）收集的评价。对于营养阶段（VS）和生殖阶段（RS），整个植物（WP）用于分析，而对于成熟阶段（MS），将植物分成不同部分进行分析，包括整个种子（WS）、种皮（SC）、子叶（Cot）和豆荚（Pod）。通过总酚和总黄酮含量（TPC和TFC）、铁还原/抗氧化能力（FRAP）和2，2-二苯基-1-苦肼基（DPPH）自由基清除试验评价了其抗氧化能力。结果表明，当考虑植物的不同部位或阶段时，同一基因型具有不同的抗氧化特性。两种总酚含量高（>92.85 mg没食子酸当量（GAE）/g干植物）和黄酮类化合物含量（>11.87 mg芦丁当量（RE）/g干植株），其抗氧化活性最高（FRAP值>= 1.157 mmol/g，DPPH自由基清除率>43.49%），可作为天然抗氧化食品。对于成熟阶段，TPC，TFC，FRAP和DPPH清除能力的最高值观察到的豆荚和最低的子叶。本研究的结果表明，蚕豆是一个很好的天然抗氧化剂的来源，独立于他们的基因型。根据阶段和植物部分的抗氧化能力的量化可以适用于与蚕豆食品产品的生产和保存相关的食品工业上的应用。(C)2011爱思唯尔有限公司保留所有权利。
In vitro antioxidant capacity of thirteen genotypes of faba bean (Vicia faba L.) collected during three different growing stages (vegetative, reproductive and maturity) have been evaluated. For the vegetative stage (VS) and reproductive stage (RS) the whole plant (WP) has been used for the analysis while for the maturity stage (MS) the plant was divided and analyzed in different parts, the whole seed (WS), seed coat (SC), cotyledons (Cot) and pod (Pod). The antioxidant capacity has been evaluated by means of the total phenolic and total flavonoid contents (TPC and TFC respectively), the ferric-reducing/antioxidant power (FRAP) and the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assays. The results showed different antioxidant properties for the same genotype when considering the different parts or stages of the plant. High content of both total phenolics (>92.85 mg as gallic acid equivalent (GAE)/g of dried plant) and flavonoids contents (>11.87 mg as rutin equivalent (RE)/g of dried plant) were found for all genotypes during the vegetative and the reproductive stages, which also showed the highest antioxidant activity (FRAP value >= 1.157 mmol/g and DPPH radical-scavenging capacity >43.49%), indicating their potential as natural sources of antioxidant foods. For the maturity stage, the highest values for the TPC,TFC, FRAP and DPPH scavenging capacity were observed for the pods and the lowest for the cotyledons.The results of this study indicate that faba beans are a good source of natural antioxidants independently to their genotype. The quantification of the antioxidant capacity according to the stage and the plant part could be suitable for applications on the food industry in relation to production and preservation of faba bean food products. (C) 2011 Elsevier Ltd. All rights reserved.