Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus)

Seasonal variations in microbial diversity and metabolite profiles of the gut of sea cucumber (Apostichopus japonicus)
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海参肠道微生物多样性和代谢物谱的季节变化

DOI:
10.3389/fmars.2022.953388
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发表时间:
2022-11
影响因子:
3.7
通讯作者:
Libin Zhang
Libin Zhang
中科院分区:
生物学2区
文献类型:
--
作者:
Beini Deng;Xiaoshang Ru;Ting Wang;Chenxi Zhang;Wanhui Sun;Songchong Lu;Libin Zhang

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海参(Apostichopus japonicus)是中国的主要经济物种,在水产养殖中具有重要作用。肠道菌群组成与外界环境密切相关。在本研究中,我们确定了季节变化对日本刺参肠道内共生微生物组成和主要代谢物的影响。我们利用16S rRNA测序技术鉴定了不同季节共生微生物的组成。采用液相色谱串联质谱法测定肠道代谢物,将共生微生物与肠道代谢物联系起来。分析不同季节肠道微生物组成的变化。结果表明,肠道微生物的季节变化显著,夏季日本刺参易受弧菌感染,豆甾醇和谷甾醇对日本刺参体壁生长有影响。研究结果对日本刺参的生长、免疫和养殖具有重要意义。
The sea cucumber (Apostichopus japonicus) is the main economic species in China and has a significant role in aquaculture. Gut microbiome composition is closely related to external environments. In this study, we identified the effects of seasonal changes on the composition and main metabolites of symbiotic microorganisms in the intestine of A. japonicus. We used 16S rRNA sequencing to identify the composition of symbiotic microorganisms in different seasons. Intestinal metabolites were determined using liquid chromatography with tandem mass spectrometry, which linked symbiotic microorganisms to intestinal metabolites. Analyzing changes in intestinal microbial composition across different seasons. The results showed that seasonal changes of intestinal microorganisms were significant, A. japonicus were infected by Vibrio easily in summer, Stigmasterol and sitosterol could affect the growth of body wall of A. japonicus. It is vital importance for A. japonicus that the results benefit for the growth, immunity and aquaculture.
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