Separation of rare oligodendrocyte progenitor cells from brain using a high-throughput multilayer thermoplastic-based microfluidic device
Separation of rare oligodendrocyte progenitor cells from brain using a high-throughput multilayer thermoplastic-based microfluidic device
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DOI：
10.1016/j.biomaterials.2013.04.014
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发表时间：
2013-07-01
期刊：
影响因子：
14
通讯作者：
Tabrizian, Maryam
中科院分区：
文献类型：
--
作者：
Didar, Tohid Fatanat;Li, Kebin;Tabrizian, Maryam
尽管再生医学领域取得了进展，但分离靶向治疗细胞的尖端技术进展仍处于早期发展阶段。这些细胞通常是稀有的，如干细胞或祖细胞，在分离过程中应保持其整体特性，以便随后应用于再生医学。这项工作，提出了分离少突胶质前体细胞(OPC)从大鼠脑原代培养使用集成热塑性弹性体(TPE)为基础的多层微流控装置，使用热压技术制造。OPC常用于中枢神经系统损伤后的恢复、修复和再生。事实上，它们在体外分化为有髓鞘的少突胶质细胞的能力，对髓鞘修复极其重要。OPC占神经胶质细胞总数的5%-10%。传统的大规模OPC分离技术需要细胞的前处理和/或多个耗时的步骤，效率低，导致其性质经常发生变化。所提出的方法意味着根据OPC的大小从脑组织混合物中分离出比其他细胞更小的OPC。该微流控芯片嵌入了5微米的膜孔径和微泵系统，分离效率可达99%以上。该芯片能够以高达100亩L/分钟的速度工作，能够在不到10分钟的时间内从汇合的75厘米(2)细胞培养瓶中分离OPC，这为我们提供了任何细胞分选技术所预期的高通量和高效分离。(C)2013爱思唯尔有限公司。保留所有权利。
Despite the advances made in the field of regenerative medicine, the progress in cutting-edge technologies for separating target therapeutic cells are still at early stage of development. These cells are often rare, such as stem cells or progenitor cells that their overall properties should be maintained during the separation process for their subsequent application in regenerative medicine. This work, presents separation of oligodendrocyte progenitor cells (OPCs) from rat brain primary cultures using an integrated thermoplastic elastomeric (TPE)- based multilayer microfluidic device fabricated using hot-embossing technology. OPCs are frequently used in recovery, repair and regeneration of central nervous system after injuries. Indeed, their ability to differentiate in vitro into myelinating oligodendrocytes, are extremely important for myelin repair. OPCs form 5-10% of the glial cells population. The traditional macroscale techniques for OPCs separation require pre-processing of cells and/or multiple time consuming steps with low efficiency leading very often to alteration of their properties. The proposed methodology implies to separate OPCs based on their smaller size compared to other cells from the brain tissue mixture. Using aforementioned microfluidic chip embedded with a 5 mu m membrane pore size and micropumping system, a separation efficiency more than 99% was achieved. This microchip was able to operate at flow rates up to 100 mu l/min, capable of separating OPCs from a confluent 75 cm(2) cell culture flask in less than 10 min, which provides us with a high-throughput and highly efficient separation expected from any cell sorting techniques. (C) 2013 Elsevier Ltd. All rights reserved.