Enhanced expression of intracellular adhesion molecule-1 and P-selectin in the diabetic human retina and choroid.
Enhanced expression of intracellular adhesion molecule-1 and P-selectin in the diabetic human retina and choroid.
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发表时间：
1995-09
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通讯作者：
D. Mcleod;D. Lefer;C. Merges;G. Lutty
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作者：
D. Mcleod;D. Lefer;C. Merges;G. Lutty
细胞间粘附分子-1 （ICAM-1）以及E-和p -选择素的表达升高发生在许多疾病状态下的血管内皮上，并且被认为在循环白细胞粘附到内皮的早期关键作用。本研究的目的是研究这些分子在非糖尿病和糖尿病患者死后视网膜和脉络膜组织切片中的免疫定位。而ICAM-1主要定位于非糖尿病受试者的绒毛膜毛细血管内，糖尿病患者的免疫反应性在整个脉络膜血管和视网膜血管内显著升高（P < 0.05）。在脉络膜中，P-选择素在非糖尿病患者的静脉中最为突出，而在糖尿病患者中，P-选择素在动脉（P < 0.001）和静脉（P < 0.05）中显著升高，在某些情况下，也在绒毛膜毛细血管中观察到P-选择素。p -选择素的免疫反应性未在任何受试者的视网膜中观察到。在所有受试者的脉络膜或视网膜中均未观察到e -选择素的免疫反应性。糖尿病脉络膜和视网膜的每平方毫米组织中性粒细胞数量显著升高（P < 0.05）。我们的研究结果表明，ICAM-1和p -选择素在正常脉络膜中组成性表达，在糖尿病患者脉络膜血管中表达上调，但在糖尿病患者的视网膜中只有ICAM-1表达上调。细胞粘附分子表达的增加可能通过增强白细胞粘附，从而导致毛细血管阻塞和内皮细胞损伤，从而导致糖尿病患者的视网膜和脉络膜微血管病变。
Elevated expression of intercellular adhesion molecule-1 (ICAM-1) as well as E- and P-selectin occurs on the vascular endothelium in a number of disease states and is thought to play an early critical role in the adhesion of circulating leukocytes to the endothelium. The goal of the present study was to investigate the immunolocalization of these molecules in the retina and choroid of postmortem human tissue sections from nondiabetic and diabetic subjects. Whereas ICAM-1 was localized primarily within the choriocapillaris of nondiabetic subjects, immunoreactivity in diabetics was significantly elevated throughout the choroidal vasculature and within retinal blood vessels (P < 0.05). In the choroid, P-selectin was most prominent in veins of the nondiabetic, whereas in diabetics, P-selectin was significantly elevated in arteries (P < 0.001) and veins (P < 0.05) and, in some cases, was also observed in choriocapillaris. P-selectin immunoreactivity was not observed in the retina of any subject. E-selectin immunoreactivity was not observed in choroid or retina in any subjects. Neutrophil numbers per square millimeter of tissue were significantly elevated in diabetic choroid (P < 0.05) and retina (P < 0.001). Our results demonstrate that ICAM-1 and P-selectin are constitutively expressed in the normal choroid and are upregulated in the choroidal vasculature in diabetes, but only ICAM-1 was upregulated in the retina of diabetic subjects. Increased cell adhesion molecule expression may contribute to the retinal and choroidal microangiopathy observed in diabetics by enhancing leukocyte adhesion and consequently the incidence of capillary obstruction and endothelial cell injury.