Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.)
Construction and characterization of a live, attenuated esrB mutant of Edwardsiella tarda and its potential as a vaccine against the haemorrhagic septicaemia in turbot, Scophthamus maximus (L.)
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迟缓爱德华氏菌活减毒 esrB 突变体的构建和表征及其作为大菱鲆出血性败血症疫苗的潜力
DOI：
10.1016/j.fsi.2006.11.002
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发表时间：
2007-09-01
影响因子：
4.7
通讯作者：
Jun, Zhang Pei
中科院分区：
文献类型：
--
作者：
Lan, Mo Zhao;Peng, Xiao;Jun, Zhang Pei
用非标记缺失法将编码III型分泌系统调节蛋白的迟缓爱德华氏菌ESRB基因突变，并通过等位基因交换的方法将其重新导入迟钝爱德华氏菌LSE40的染色体上。LSE40ESRB突变体经腹腔接种大比目鱼后，其致死量为10(8.1)cfu/鱼，致死率为50%。在接种后14天，ESRB突变体不能从内脏中恢复。单次接种10(5)-10(7)CFU/FISH的ESRB突变体对野生型迟缓E.tarda LSE40株有明显的保护作用(相对存活率为50%)。免疫效果与免疫鱼血清中的抗体效价有很好的相关性。(C)2006爱思唯尔有限公司。保留所有权利。
The esrB gene of Edwardsiella tarda, which encodes a regulator protein of the type III secretion system, was mutated by the unmarked deletion method and reintroduced by allelic exchange into the chromosome of E. tarda LSE40 by means of the suicide vector pRE 112. The LSE40 esrB mutant was highly attenuated when inoculated intraperitoneally into turbot Scophthamus maximus L., showing a 50% lethal dose of 10(8.1) cfu/fish. The esrB mutants were not recoverable from the internal organs at 14 days post-inoculation. Vaccination with a single dose of 10(5)-10(7) cfu/fish of the esrB mutant elicited significant protection against the wildtype strain of E. tarda LSE40 (relative percentage survival > 50%). The protection correlated well with the antibody titres in the serum of vaccinated fish. (c) 2006 Elsevier Ltd. All rights reserved.