Cross-basin comparison of phosphorus stress and nitrogen fixation in Trichodesmium
Cross-basin comparison of phosphorus stress and nitrogen fixation in Trichodesmium
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DOI：
10.4319/lo.2009.54.5.1438
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发表时间：
2009-09-01
影响因子：
4.5
通讯作者：
Webb, Eric A.
中科院分区：
文献类型：
--
作者：
Hynes, Annette M.;Chappell, P. Dreux;Webb, Eric A.
我们调查了来自北太平洋、西南太平洋和西北大西洋的束毛藻种群的磷（P）状况和固氮率。收集束毛藻属的菌落，并使用酶标记荧光（ELF）分析内源性碱性磷酸酶（AP）活性和使用乙炔还原分析固氮酶活性。AP从溶解的有机磷中水解溶解的无机磷酸盐（DIP），并且在经历P胁迫的束毛藻菌落中具有活性。在亚热带北太平洋和南太平洋的多个站点中，束毛藻中存在低至中度的ELF标记，尽管其他类群中存在标记。相反，在北大西洋束毛藻ELF标记从低到高不等。低ELF标记对应于高DIP浓度，而高ELF标记仅发生在具有DIP浓度的北大西洋站点
We investigated the phosphorus (P) status and N-2 fixation rates of Trichodesmium populations from the North Pacific, western South Pacific, and western North Atlantic. Colonies of Trichodesmium were collected and analyzed for endogenous alkaline phosphatase (AP) activity using enzyme-labeled fluorescence ( ELF) and for nitrogenase activity using acetylene reduction. AP hydrolyzes dissolved inorganic phosphate (DIP) from dissolved organic phosphorus and is active in Trichodesmium colonies experiencing P stress. Across multiple stations in the subtropical North and South Pacific, there was low to moderate ELF labeling in Trichodesmium, although labeling was present in other taxa. In contrast, Trichodesmium ELF labeling in the North Atlantic ranged from low to high. Low ELF labeling corresponded with high DIP concentrations while high ELF labeling occurred only at North Atlantic stations with DIP concentrations