ALIGNMENT PHYLOGENY OF THE PAPAIN SUPERFAMILY OF CYSTEINE PROTEASES
ALIGNMENT PHYLOGENY OF THE PAPAIN SUPERFAMILY OF CYSTEINE PROTEASES
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DOI：
10.1006/jmbi.1994.0083
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发表时间：
1995-02-17
影响因子：
5.6
通讯作者：
STORER, AC
中科院分区：
文献类型：
--
作者：
BERTI, PJ;STORER, AC
使用基于初始结构的比对，然后连续迭代序列比对和系统发育推断，创建了半胱氨酸蛋白酶木瓜蛋白酶超家族的比对/系统发育。迭代方法导致比对/系统发育的显着改善。有三组半胱氨酸蛋白酶，它们关系较远，只能在活性位点区域相互比对：木瓜蛋白酶组，包括诸如组织蛋白酶 B、C、H、L 和 S 等典型的半胱氨酸蛋白酶；以及博莱霉素水解酶和钙蛋白酶组。博来霉素水解酶和钙蛋白酶组各有一个细菌序列。前者可能是由横向基因转移引起的，后者可能是由早于真核生物/原核生物分歧的祖先蛋白酶直接进化而来的。木瓜蛋白酶类群的系统发育表明，在真核进化的早期，许多家族几乎同时分化。哺乳动物中至少有 12 个不同的半胱氨酸蛋白酶家族，甚至可能更多，其中包括至少两种尚未表征的酶。
An alignment/phylogeny of the papain superfamily of cysteine proteases was created using an initial structure-based alignment followed by successive iterations of sequence alignment and phylogenetic inference. The iterative approach resulted in significant improvements in the alignment/phylogeny There were three groups of cysteine proteases that were distantly related and which could be aligned against each other only in the active site regions: the papain group, which included such stereotypical cysteine proteases as cathepsins B, C, H, L and S; and the bleomycin hydrolase and calpain groups. There was one bacterial sequence in each of the bleomycin hydrolase and calpain groups. The former probably arose by lateral gene transfer, the latter possibly by direct evolution from an ancestral protease predating the eukaryote/prokaryote divergence. The phylogeny of the papain group indicated that many families diverged almost simultaneously early during eukaryotic evolution. In mammals there are at least 12 distinct families of cysteine proteases, possibly many more, including at least two as yet uncharacterized enzymes.