Genotypic diversity and genetic structure of populations of the distylous aquatic plant Nymphoides peltata (Menyanthaceae) in China
Genotypic diversity and genetic structure of populations of the distylous aquatic plant Nymphoides peltata (Menyanthaceae) in China
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中国荇菜科水生植物种群基因型多样性及遗传结构
DOI：
10.1111/jse.12023
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发表时间：
2013-09-01
影响因子：
3.7
通讯作者：
Chen, Jin-Ming
中科院分区：
文献类型：
--
作者：
Liao, Yi-Ying;Yue, Xiao-Li;Chen, Jin-Ming
菜是一种二生水生植物。为了探讨克隆生长和有性繁殖在居群中的相对重要性，揭示我国常见的单花柱变型居群的起源，对该种的克隆多样性和居群遗传结构进行了研究。通过使用7个单序列重复引物对，共192个多位点基因型（MLG）的472个样本中，从21个分析的人口，每个人口有两个或两个以上的MLG。单花柱型群体的MLG数量低于二花柱型群体。分子变异分析表明，居群间遗传变异所占比例较小（36.8%）。邻接树分析表明，居群间的遗传变异不存在明显的地理结构。盾形。瓶颈测试表明，在大多数研究人群中存在重大瓶颈。从种子中成功的有性补充可能是造成N.中国的盾形贝。人口统计学随机性或瓶颈应该解释具有多个MLG的一种风格变体群体。同时，由于人类活动在种群中的随机发生，导致了瓶颈效应或种群数量的变化，这也是导致被调查种群间遗传变异没有明显地理结构的原因。盾形。
Nymphoides peltata is a distylous aquatic plant. In order to explore the relative importance of clonal growth and sexual reproduction within populations and also to reveal the origin of the commonly occurring one style morph populations in China, we studied the clonal diversity and population genetic structure of this species. By using seven single sequence repeat primer pairs, a total of 192 multilocus genotypes (MLGs) were identified among the 472 samples from the 21 populations analyzed; each population had two or more MLGs. The number of MLGs was lower for the one style morph populations than distylous populations. Analysis of molecular variance showed that a smaller proportion of genetic variation resided among populations (36.8%). A neighbor‐joining tree indicated that there was no significant geographical structure of the genetic variation among populations of N. peltata. The Bottleneck tests indicated that there had been significant bottlenecks in most of the studied populations. The successful sexual recruitment from seeds may have contributed to the high clonal diversity in distylous populations of N. peltata in China. Demographic stochasticity or bottleneck should account for the one style morph population with several MLGs. And also the bottleneck effects or demographic changes caused by human activities occurring randomly in populations should account for the finding of no significant geographical structure of the genetic variation among the investigated populations of N. peltata.