Design and Performance of the CDC Real-Time Reverse Transcriptase PCR Swine Flu Panel for Detection of 2009 A (H1N1) Pandemic Influenza Virus
Design and Performance of the CDC Real-Time Reverse Transcriptase PCR Swine Flu Panel for Detection of 2009 A (H1N1) Pandemic Influenza Virus
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DOI：
10.1128/jcm.02636-10
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发表时间：
2011-07-01
影响因子：
9.4
通讯作者：
Lindstrom, Stephen
中科院分区：
文献类型：
--
作者：
Shu, Bo;Wu, Kai-Hui;Lindstrom, Stephen
猪流感病毒 (SIV) 已被证明可以零星感染人类，并且在收到不可分型的甲型流感病毒样本后，美国疾病控制与预防中心 (CDC) 流感部门很少对其进行鉴定。 CDC 开发并实施了用于检测和表征北美谱系 (N. Am) SIV 的实时逆转录酶 PCR (rRT-PCR) 程序，以快速识别疑似 SIV 感染病例的标本。这些程序于 2009 年 4 月用于检测 2009 年甲型 (H1N1) 大流行 (pdm) 流感病毒感染的人类病例。根据前两种研究病毒的基因序列数据，对先前开发的 rRT-PCR 程序进行了优化，创建了 CDC rRT-PCR 猪流感面板，用于检测 2009 A (H1N1) pdm 流感病毒。 CDC rRT-PCR 猪流感小组的分析灵敏度为每个反应 5 个 RNA 拷贝，对于培养病毒每个反应 10(-1.3 类似于 -0.7) 50% 感染剂量 (ID(50))。在检测人类季节性甲型（H1N1、H3N2）和乙型流感病毒阳性的人类临床标本或培养病毒时，未观察到交叉反应。 2009 年 4 月至 2010 年 6 月，CDC rRT-PCR 猪流感检测试剂盒分发给美国和国际公共卫生实验室。CDC rRT-PCR 猪流感检测试剂盒是及时、特异性检测 2009 A (H1N1) pdm 流感病毒的有效工具，并促进了后续公共卫生应对措施的实施。
Swine influenza viruses (SIV) have been shown to sporadically infect humans and are infrequently identified by the Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypeable influenza A virus samples. Real-time reverse transcriptase PCR (rRT-PCR) procedures for detection and characterization of North American lineage (N. Am) SIV were developed and implemented at CDC for rapid identification of specimens from cases of suspected infections with SIV. These procedures were utilized in April 2009 for detection of human cases of 2009 A (H1N1) pandemic (pdm) influenza virus infection. Based on genetic sequence data derived from the first two viruses investigated, the previously developed rRT-PCR procedures were optimized to create the CDC rRT-PCR Swine Flu Panel for detection of the 2009 A (H1N1) pdm influenza virus. The analytical sensitivity of the CDC rRT-PCR Swine Flu Panel was shown to be 5 copies of RNA per reaction and 10(-1.3 similar to-0.7) 50% infectious doses (ID(50)) per reaction for cultured viruses. Cross-reactivity was not observed when testing human clinical specimens or cultured viruses that were positive for human seasonal A (H1N1, H3N2) and B influenza viruses. The CDC rRT-PCR Swine Flu Panel was distributed to public health laboratories in the United States and internationally from April 2009 until June 2010. The CDC rRT-PCR Swine Flu Panel served as an effective tool for timely and specific detection of 2009 A (H1N1) pdm influenza viruses and facilitated subsequent public health response implementation.