The effect of feeding carrots on immunoglobulin E production and anaphylactic response in mice.
The effect of feeding carrots on immunoglobulin E production and anaphylactic response in mice.
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喂食胡萝卜对小鼠免疫球蛋白 E 产生和过敏反应的影响。
DOI：
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发表时间：
1999
影响因子：
2
通讯作者：
M. Toyoda
中科院分区：
文献类型：
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作者：
H. Akiyama;K. Hoshino;M. Tokuzumi;R. Teshima;H. Mori;T. Inakuma;Y. Ishiguro;Y. Goda;J. Sawada;M. Toyoda
将胡萝卜汁口服给用二硝基苯化（DNP）-OVA腹腔内免疫的BALB/c小鼠约1个月。测定小鼠血清中DNP特异性IgE、DNP特异性IgG的滴度和总IgE水平。胡萝卜汁能显著抑制小鼠产生DNP特异性IgE。另一方面，DNP特异性IgG的生产和总IgE水平的小鼠喂食胡萝卜汁没有显着差异，在对照组小鼠。我们还研究了喂胡萝卜对速发型超敏反应的影响。抗原刺激后一小时，喂食胡萝卜的小鼠耳朵肿胀程度低于对照小鼠。此外，在主动全身过敏反应下，喂食胡萝卜的小鼠血清组胺的升高低于对照组。然后，我们研究了由小鼠脾细胞产生细胞因子的模式，然后在体外用DNP-OVA再刺激。喂食胡萝卜的小鼠的脾细胞比对照组产生更多的干扰素-γ。相比之下，喂食胡萝卜的小鼠的脾细胞产生的白细胞介素-4比对照组少。此外，白细胞介素-12的产生也高于对照组。这些发现表明，喂食胡萝卜改善了辅助性T细胞（Th）1/Th 2平衡，抑制了特异性IgE的产生和抗原诱导的过敏反应。
Carrot juice was administered orally to BALB/c mice immunized intraperitoneally with dinitrophenylated (DNP)-OVA for about 1 month. The titers of DNP-specific IgE, DNP-specific IgG, and the levels of total IgE in mouse sera were determined. The DNP-specific IgE production by mice fed carrot juice was significantly inhibited. On the other hand, the DNP-specific IgG production and the level of total IgE in mice fed carrot juice were not significantly different from those in control mice. We also examined the effect of feeding carrots on immediate-type hypersensitivity. One hour after antigen stimulation, the ears of mice fed carrots swelled less than those of control mice. Furthermore, the rise in serum histamine in the mice fed carrots under active systemic anaphylaxis was lower than in controls. We then examined the pattern of cytokine production by spleen cells from mice followed by restimulation with DNP-OVA in vitro. The spleen cells from the mice fed carrots produced more interferon-gamma than those from the control group. In contrast, the spleen cells from the mice fed carrots produced less interleukin-4 than those from the control group. Furthermore, the interleukin-12 production of the spleen cells from mice fed carrots was also higher than that of the control group. These findings suggest that feeding carrots improves the helper T cell (Th)1/Th2 balance, inhibiting specific IgE production and antigen-induced anaphylactic response.