Application of Phytoplankton
Application of Phytoplankton
复制标题
浮游植物的应用
DOI：
10.1007/978-4-431-55957-3_19
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Toshiyuki Takahashi
中科院分区：
文献类型：
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作者：
Toshiyuki Takahashi
本章介绍了浮游植物和小球藻作为模式生物及其在金属毒性评价中的应用。根据JIS K 0058-1的浸出条件，从钢渣中浸出用于评价金属毒性的金属溶液。本研究还探讨了各种方法的发展，这些方法不仅可以方便地估计藻类的数量，还可以方便地估计藻类的活动。经测试的炉渣中金属流出物的浓度几乎都低于流出物和饮用水的环境质量标准。将小球藻与含有本研究中所测试的矿渣洗脱液的培养基孵育后，使用显微镜和流式细胞术研究每种洗脱液的作用。对每种洗脱液处理过的藻类细胞的分析显示，洗脱液既不致死性也不抑制生长。除了显微细胞计数外，利用流式细胞术进行生物测定，同时估计旺盛和异常的藻类生长。而不是表现出细胞毒性的钢渣洗脱液的加入，促进藻类的生长。
This chapter describes the phytoplanktonChlorellaspecies as model organisms and their application to metal toxicity evaluation. Metallic solutions used to assess metal toxicity were eluted from steel slag with leaching conditions based on JIS K 0058-1. This study also examined the development of methods to easily estimate not only the number of algae but also their algal activities. The concentrations of metal effluents from tested slags were almost all lower than environmental quality standards for effluent and drinking water. After incubation ofChlorellawith culture media including eluates from the respective slags tested in this study, the effects of each eluate were investigated using microscopy and flow cytometry. Analyses of algal cells treated with each eluate revealed that eluate induced neither lethality nor growth inhibition. In addition to microscopic cell counting, bioassay using flow cytometry was used to estimate vigorous and aberrant algal growth simultaneously. Instead of exhibiting cytotoxicity, the addition of each eluate from steel slag enhanced algal growth.