Synthesis of Tn/T Antigen MUC1 Glycopeptide BSA Conjugates and Their Evaluation as Vaccines
Synthesis of Tn/T Antigen MUC1 Glycopeptide BSA Conjugates and Their Evaluation as Vaccines
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DOI：
10.1002/ejoc.201100304
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发表时间：
2011-07-01
影响因子：
2.8
通讯作者：
Li, Yan-Mei
中科院分区：
文献类型：
--
作者：
Cai, Hui;Huang, Zhi-Hua;Li, Yan-Mei
肿瘤相关粘蛋白MUC 1在大多数上皮肿瘤组织中过表达，被认为是一个有前途的免疫治疗靶点。MUC 1的细胞外部分含有氨基酸序列HGVTSAPDTRPAPGSTAPPA的许多串联重复的结构域，包括五个潜在的O-糖基化位点。本研究选择T9和S15作为糖基化位点。采用微波辅助Fmoc固相肽合成法合成了N-末端带有三甘醇间隔基的糖肽。从树脂上分离并脱保护后，将MUC 1糖肽与牛血清白蛋白（BSA）偶联。为了评估免疫学特性，用这些BSA疫苗免疫balb/c小鼠。
The tumor-associated mucin MUC1 over-expressed in most epithelial tumor tissues is considered a promising target for immunotherapy. The extracellular part of MUC1 contains a domain of numerous tandem repeats of the amino acid sequence HGVTSAPDTRPAPGSTAPPA, including five potential O-glycosylation sites. In this study, T9 and S15 have been chosen as the positions of glycosylation. The glycopeptides N-terminally equipped with a triethylene glycol spacer were synthesized by microwave-assisted Fmoc solid-phase peptide synthesis. After detachment from the resin and deprotection, the MUC1 glycopeptides were conjugated to bovine serum albumin (BSA). To evaluate the immunological properties, balb/c mice were immunized with these BSA vaccines.