Supercritical CO2 extraction of carotenoids (astaxanthin, adonirubin, and adonixanthin) from Paracoccus carotinifaciens: Improved Z‐isomer ratio and recovery of carotenoids via high‐temperature extraction
Supercritical CO2 extraction of carotenoids (astaxanthin, adonirubin, and adonixanthin) from Paracoccus carotinifaciens: Improved Z‐isomer ratio and recovery of carotenoids via high‐temperature extraction
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超临界 CO2 从胡萝卜副球菌中提取类胡萝卜素（虾青素、金盏花红素和金盏花黄素）：通过高温提取提高 Z 异构体比例并回收类胡萝卜素
DOI：
10.1002/ejlt.202200021
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发表时间：
2022
影响因子：
2.7
通讯作者：
Kuroda Ikuo
中科院分区：
文献类型：
--
作者：
Honda Masaki;Murakami Kazuya;Osawa Yukiko;Kawashima Yuki;Wasai Masafumi;Hirasawa Kazuaki;Kuroda Ikuo
研究了高温超临界CO2（SC-CO2）萃取对胡萝卜素副球菌（Paracoccus carotinifaciens）中类胡萝卜素（虾青素、侧金盏花红色素和侧金盏花黄素）Z-异构化和回收的影响。高温提取，特别是在>100 °C下，不仅增加了所得提取物中类胡萝卜素的总Z-异构体比率，而且还提高了类胡萝卜素的回收率。例如，当在60 °C和30 MPa下用萃取剂（乙醇）进行SC-CO2萃取时，总Z-异构体比率和虾青素的回收率分别为11.1%和28.4%，而在120 °C下，该值分别增加到54.2%和56.4%。在高温下类胡萝卜素的提取效率的显着改善可以归因于在SC-CO2中具有高溶解度的类胡萝卜素Z-异构体的比例的增加。此外，还研究了萃取压力和萃取剂含量对Z-异构化和类胡萝卜素回收率的影响。结果表明，较高的压力和乙醇含量导致较低的Z-异构体比率和较高的类胡萝卜素回收率。由于类胡萝卜素的Z-异构体具有更高的生物利用度，并且可能比所有E-异构体表现出上级的生物活性，高附加值的类胡萝卜素可以同时通过高温提取获得更高的产量。实际应用：由于类胡萝卜素提取通常使用有毒有机溶剂进行，因此残留溶剂是一个严重的问题。此外，天然存在的类胡萝卜素（主要是全E异构体）具有低生物利用度。这些都是类胡萝卜素的工业应用需要克服的重要问题。在高温条件下通过SC-CO2萃取获得的富含Z-异构体的类胡萝卜素具有同时解决这些问题的巨大潜力。
The effects of high‐temperature supercritical CO2(SC‐CO2) extraction onZ‐isomerization and recovery of carotenoids (astaxanthin, adonirubin, and adonixanthin) fromParacoccus carotinifaciensare investigated. The high‐temperature extraction, especially at >100 °C, not only increases the totalZ‐isomer ratio of carotenoids in the resulting extracts but also improves carotenoid recovery. For example, when the SC‐CO2extraction is performed at 60 °C and 30 MPa with an entrainer (ethanol), the totalZ‐isomer ratio and the recovery of astaxanthin are 11.1% and 28.4%, respectively, whereas at 120 °C, the values increase to 54.2 and 56.4%, respectively. The significant improvement in the extraction efficiency of carotenoids at high temperatures can be attributed to the increase in the ratio of carotenoidZ‐isomers with high solubility in SC‐CO2. Moreover, the effects of extraction pressure and entrainer content onZ‐isomerization and recovery of carotenoids are also investigated. The results show that higher pressure and ethanol content resulted in lowerZ‐isomer ratios and higher recoveries of carotenoids. As theZ‐isomers of carotenoids have greater bioavailability and potentially exhibit superior biological activities than the all‐E‐isomers, high value‐added carotenoids, and higher yield can be simultaneously achieved with high‐temperature extraction.Practical applications: As carotenoid extraction is usually performed using toxic organic solvents, residual solvents are a serious concern. In addition, naturally occurring carotenoids (mainly the all‐E‐isomers) have low bioavailability. These are important issues that need to be overcome for the industrial use of carotenoids. TheZ‐isomer‐rich carotenoids obtained by SC‐CO2extraction under high‐temperature conditions have great potential to solve these issues simultaneously.