DNA DAMAGE AND REPAIR FOLLOWING TREATMENT OF V-79 CELLS WITH SULFONATED PHTHALOCYANINES
DNA DAMAGE AND REPAIR FOLLOWING TREATMENT OF V-79 CELLS WITH SULFONATED PHTHALOCYANINES
复制标题
DOI：
10.1111/j.1751-1097.1987.tb07880.x
复制
发表时间：
1987-06-01
影响因子：
3.3
通讯作者：
VANLIER, JE
中科院分区：
文献类型：
--
作者：
HUNTING, DJ;GOWANS, BJ;VANLIER, JE
测试了两种镓酞菁对完整细胞和渗透性细胞中细胞存活、胰蛋白酶消化和 DNA 链断裂的影响，并通过碱性洗脱进行测量。四磺基酞菁镓对细胞没有光毒性，并且不会对完整细胞造成可测量的 DNA 损伤，而每个分子平均含有 2.7 个磺酸盐的磺化程度较低的镓磺基酞菁混合物具有很强的光毒性，并会诱导 DNA 链断裂和/或碱敏感位点；然而，这两种药物在诱导通透性细胞中 DNA 链断裂方面同样有效。 DNA损伤在完整的细胞中迅速修复；在 DNA 复制合成被抑制超过 90% 的条件下，这一过程不受阿菲迪霉素（DNA 聚合酶 α 和 δ 的抑制剂）的抑制。
Two gallium-phthalocyanines were tested for their effects on cell survival, trypsinization, and DNA strand breaks in intact and permeable cells, measured by alkaline elution. Gallium-tetrasulfophthalocyanine was not phototoxic to cells and caused no measurable DNA damage in intact cells, while a mixture of less sulfonated gallium-sulfophthalocyanines, containing an average of 2.7 sulfonates per molecule, was very phototoxic and induced DNA strand breaks and/or alkali-sensitive sites; however, both drugs were equally effective at inducing DNA strand breaks in permeable cells. The DNA damage was rapidly repaired in intact cells; this process was not inhibited by aphidicolin, an inhibitor of DNA polymerases alpha and delta, under conditions in which DNA replicative synthesis was inhibited by more than 90%.