MODULATION OF MESSENGER-RNA FOR MICROTUBULE-ASSOCIATED PROTEINS DURING BRAIN-DEVELOPMENT
MODULATION OF MESSENGER-RNA FOR MICROTUBULE-ASSOCIATED PROTEINS DURING BRAIN-DEVELOPMENT
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DOI：
10.1073/pnas.79.16.4892
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
LITTAUER, UZ
中科院分区：
文献类型：
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作者：
GINZBURG, I;SCHERSON, T;LITTAUER, UZ
大鼠脑中微管相关蛋白τ的异质性是由发育决定的。新生大鼠脑含有2种τ多肽（τ0），其分子量与12日龄大鼠脑微管相关的5种τ组分（τ12）略有不同。τ0和τ12具有免疫相关性，并与抗τ12蛋白的抗体发生交叉反应。通过将未分级的含多聚腺苷酸的mRNA与含有微管蛋白和肌动蛋白序列的cDNA制剂预先杂交，实现了τ mRNA的富集。剩余的未杂交mRNA通过在氢氧化甲基汞琼脂糖凝胶上进行电泳进一步分级。涉及mRNA无细胞翻译的实验表明，新生脑和发育中脑的τ蛋白组成的主要差异在mRNA水平受到控制。新生大鼠脑的mRNA指导合成5种τ蛋白，其中2种为新生脑所特有，而另外3种形式是发育中脑的特征。因此，在新生脑中，编码3种τ12形式的特异性mRNA种类的出现先于这些蛋白在细胞中的合成阶段。12日龄脑的mRNA指导合成4种发育中脑特有的τ蛋白，其中一种不由新生脑的mRNA合成。用从12日龄脑分离的mRNA没有合成任何新生的τ0形式。
The heterogeneity of .tau. microtubule-associated proteins from rat brain is developmentally determined. Newborn rat brain contains 2 .tau. polypeptides (.tau.0) with somewhat different MW than the 5 .tau. components associated with microtubules from 12-day-old brain (.tau.12). .tau.0 and .tau.12 are immunologically related and crossreact with antibodies against .tau.12 proteins. Enrichment of the .tau. mRNA was achieved by prior hybridization of unfractionated poly(A)-containing mRNA to cDNA preparations containing tubulin and actin sequences. The remaining unhybridized mRNA was further fractionated by electrophoresis on methylmercury hydroxide agarose gels. Experiments involving cell-free translation of mRNA indicated that the major differences in the composition of .tau. proteins from newborn and developing brain are controlled at the mRNA level. The mRNA from newborn rat brain directed the synthesis of 5 .tau. proteins, 2 of which are specific for newborn brain, whereas the other 3 forms are characteristic of the developing brain. Thus, the appearance in newborn brain of mRNA species specific for 3 .tau.12 forms precedes the phase of the synthesis of these proteins in the cell. mRNA from 12-day brain directed the synthesis of 4 .tau. proteins specific for the developing brain, one of which is not synthesized by mRNA from newborn brain. None of the newborn .tau.0 forms were synthesized with mRNA isolated from 12-day brain.