Interleukin 10 protects mice from lethal endotoxemia.
Interleukin 10 protects mice from lethal endotoxemia.
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白介素10可保护小鼠免受致命性内毒素血症的侵害。
DOI：
10.1084/jem.177.4.1205
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发表时间：
1993-04-01
影响因子：
15.3
通讯作者：
Menon, S
中科院分区：
文献类型：
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作者：
Howard, M;Muchamuel, T;Andrade, S;Menon, S
白细胞介素10（IL-10）在体外减少IL-1、IL-6和肿瘤坏死因子α（TNF-α）的产生，并且小鼠中IL-10的中和导致相同单核因子的升高。我们在此测试IL-10的这种单核因子抑制特性是否赋予其保护小鼠免受脂多糖诱导的休克（单核因子介导的炎症反应）的能力。单次注射0.5-1 μ g重组鼠IL-10可重复地保护BALB/c小鼠免受致死性腹膜内注射内毒素的影响。无论IL-10是与内毒素注射同时给药还是在内毒素注射后30分钟给药，均获得该结果。IL-10的保护作用通过预先注射中和性抗IL-10抗体逆转，并且与内毒素诱导的TNF-α释放的实质性降低相关。这些数据表明IL-10是治疗细菌性脓毒症的候选药物，更一般地说，是有效的抗脓毒症试剂。
Interleukin 10 (IL-10) decreases production of IL-1, IL-6, and tumor necrosis factor alpha (TNF-alpha) in vitro, and neutralization of IL-10 in mice leads to elevation of the same monokines. We test here whether this monokine-suppressing property of IL-10 confers on it the capacity to protect mice from lipopolysaccharide-induced shock, a monokine- mediated inflammatory reaction. A single injection of 0.5-1 microgram of recombinant murine IL-10 reproducibly protected BALB/c mice from a lethal intraperitoneal injection of endotoxin. This result was obtained whether the IL-10 was administered concurrently with, or 30 min after the injection of endotoxin. The protective effect of IL-10 was reversed by prior injection of neutralizing anti-IL-10 antibodies, and correlated with a substantial decrease in endotoxin-induced TNF-alpha release. These data implicate IL-10 as a candidate for treatment of bacterial sepsis, and more generally as an effective antiinflammatory reagent.