STATE TRANSITIONS IN A PHYCOBILISOME-LESS MUTANT OF THE CYANOBACTERIUM SYNECHOCOCCUS SP PCC-7002
STATE TRANSITIONS IN A PHYCOBILISOME-LESS MUTANT OF THE CYANOBACTERIUM SYNECHOCOCCUS SP PCC-7002
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DOI：
10.1016/s0005-2728(89)80166-5
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发表时间：
1989-04-17
期刊：
影响因子：
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通讯作者：
BRYANT, DA
中科院分区：
文献类型：
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作者：
BRUCE, D;BRIMBLE, S;BRYANT, DA
在蓝细菌聚球藻7002的野生型细胞和缺乏藻胆体的突变细胞中研究了状态转换。在DCMU诱导的状态1和暗适应诱导的状态2在野生型和突变体细胞的存在下，通过77 K荧光发射光谱测定的预照射。光诱导的转换后，观察到在野生型藻蓝蛋白（状态2）或优先激发叶绿素a（状态1）的优先激发。光诱导的转换也观察到藻胆体=少突变体后优先激发的短波长叶绿素a（状态2）或类胡萝卜素和长波长叶绿素a（状态1）。我们的结论是，在蓝藻的光状态转换的机制不需要存在的藻胆体。我们的研究结果与所提出的模型相矛盾的状态转换，这需要磷酸化的，和一个积极的作用，藻胆体。
State transitions were investigated in the cyanobacterium Synechococcus sp. PCC 7002 in both wild-type cells and mutant cells lacking phycobilisomes. Preillumination in the presence of DCMU induced State 1 and dark-adaptation induced State 2 in both wild-type and mutant cells as determined by 77 K fluorescence emission spectroscopy. Light-induced transitions were observed in the wild-type after preferential excitation of phycocyanin (State 2) or preferential excitation of Chl a (State 1). Light-induced transitions were also observed in the phycobilisome=less mutant after preferential excitation of short-wavelength Chl a (State 2) or carotenoids and long-wavelength Chl a (State 1). We conclude that the mechanism of the light-state transition in cyanobacteria does not require the presence of the phycobilisome. Our results contradict proposed models for the state transition, which require phosphorylation of, and an active role for, the phycobilisome.