Protein-resistant polyurethane via surface-initiated atom transfer radical polymerization of oligo(ethylene glycol) methacrylate
Protein-resistant polyurethane via surface-initiated atom transfer radical polymerization of oligo(ethylene glycol) methacrylate
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DOI：
10.1002/jbm.a.32319
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发表时间：
2009-12-15
影响因子：
4.9
通讯作者：
Brash, John L.
中科院分区：
文献类型：
--
作者：
Jin, Zhilin;Feng, Wei;Brash, John L.
通过聚（低聚（乙二醇）甲基丙烯酸酯）（聚（OEGMA））的表面引发同时正向和反向原子转移自由基聚合（s-ATRP）制备抗蛋白质聚氨酯（PU）表面。采用氧等离子体处理对 PU 表面进行初始活化。通过改变溶液中单体与牺牲引发剂的摩尔比从5:1至200:1来调节接枝聚合物链长。改性 PU 表面通过水接触角、X 射线光电子能谱 (XPS) 和原子力显微镜 (AFM) 进行表征。通过 tris 缓冲盐水 (TBS) 和血浆进行蛋白质吸附实验，以评估表面的蛋白质耐受性。修饰后，单一和二元蛋白质溶液以及血浆的吸附显着减少。吸附随着聚（OEGMA）链长度的增加而降低。 200:1 单体/引发剂表面上的纤维蛋白原 (Fg) 吸附量在 3-33 ng/cm(2) 范围内，与未改性的 PU 相比，吸附量减少了 96-99%。 Fg吸附量从0.01-10%，血浆低至1-5 ng/cm(2)。此外，使用 Fg 和溶菌酶 (Lys) 的二元蛋白质吸附实验表明，蛋白质大小是这些表面的蛋白质耐受性的一个因素。 （D 2009 Wile），期刊公司 J Biomed Mater Res 91A：1189-1201，2009
Protein-resistant polyurethane (PU) surfaces were prepared by surface-initiated simultaneous normal and reverse atom transfer radical polymerization (s-ATRP) of poly(oligo(ethylene glycol) methacrylate) (poly (OEGMA)). Oxygen plasma treatment was employed for initial activation of the PU surface. The grafted polymer chain length was adjusted by varying the molar ratio of monomer to sacrificial initiator in Solution from 5:1 to 200:1. The modified PU surfaces were characterized by water contact angle, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Protein adsorption experiments from tris-buffered saline (TBS) and plasma were carried out to evaluate the protein-resistance of the surfaces. Adsorption from single and binary protein solutions as well as from plasma was significantly reduced after modification. Adsorption decreased with increasing poly(OEGMA) chain length. Fibrinogen (Fg) adsorption on the 200:1 monomer/initiator surface was in the range of 3-33 ng/cm(2) representing 96-99% reduction compared with the unmodified PU. Fg adsorption from 0.01-10%, plasma was as low, as 1-5 ng/cm(2). Moreover, binary protein adsorption experiments using Fg and lysozyme (Lys) showed that protein size is a factor in the protein resistance of these surfaces. (D 2009 Wile), Periodicals, Inc. J Biomed Mater Res 91A: 1189-1201, 2009