Synthesis of DNA fragments containing 2’-deoxy-4’-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4’-position in replication
Synthesis of DNA fragments containing 2’-deoxy-4’-selenonucleoside units using DNA polymerases: comparison of dNTPs with O, S and Se at the 4’-position in replication
复制标题
使用 DNA 聚合酶合成含有 2-脱氧-4-硒核苷单元的 DNA 片段：复制中 4 位 dNTP 与 O、S 和 Se 的比较
DOI：
10.1039/c5ob00941c
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Tatsuhiro Ishida and Noriaki Minakawa*
中科院分区：
文献类型：
--
作者：
Noriko Tarashima;Tatsuya Sumitomo;Hidenori Ando;Kazuhiro Furukawa;Tatsuhiro Ishida and Noriaki Minakawa*
利用4′-硒胸苷三磷酸（SeTTP）对DNA聚合酶的生物等效性，首次合成了4′-硒脱氧核糖核酸（4′-SelenoDNA）片段。使用KOD Dash DNA聚合酶有效地扩增了在胸苷单元的4′-位置处各自具有同源元件（O、S或Se）的DNA片段。
4′-SelenoDNA fragments were synthesized for the first time using 4′-selenothymidine triphosphate (SeTTP) by taking advantage of its bioequivalence against DNA polymerases. DNA fragments each with a homologous element (O, S or Se) at the 4′-position of the thymidine units were effectively amplified using KOD Dash DNA polymerase.