Fundamental cellular heterogeneity of the exocrine pancreas
Fundamental cellular heterogeneity of the exocrine pancreas
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DOI：
10.1177/44.3.8648080
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发表时间：
1996-03-01
影响因子：
3.2
通讯作者：
Adelson, JW
中科院分区：
文献类型：
--
作者：
Jeraldo, TL;Coutu, JA;Adelson, JW
结构和功能的均一性被广泛认为是胰腺腺泡消化酶分泌组织的特征。近年来，生理学研究表明，胰腺将消化酶储存在异质组成的池中，并以周期性和促分泌素特异性的方式从这些池中释放消化酶。胰腺异质性的细胞基础是未知的，经典的光镜和电镜制备似乎均匀，我们应用了一组生物素化凝集素的胰腺组织切片，腺泡细胞糖缀合物定位在原位与过氧化物酶和荧光技术和莱宾金络合物。禁食家兔和大鼠胰腺的凝集素结合特性揭示了腺泡细胞群的广泛和特异性异质性。光镜和电镜显示用凝集素荆豆凝集素（UEA）和刺桐凝集素（EGA）高度异质性标记特定腺泡细胞的酶原颗粒内容物，其还显示岛周腺泡的特异性标记，其他凝集素也表现出异质性结合到特定的细胞区域。引人注目的腺泡细胞异质性证实了早期的预测，并可能最终被证明是胰腺内异质来源的不同酶混合物分泌的细胞基础。
Homogeneity in structure and function are broadly assumed to be characteristics of the acinar pancreatic digestive enzyme-secreting tissue, In recent years, physiological studies have shown that the pancreas stores the digestive enzymes in heterogeneously composed pools and releases them from these pools in a cyclic and secretagogue-specific fashion. The cellular basis for pancreatic heterogeneity is unknown; classical light and electron microscopic preparations appear homogeneous, We applied a panel of biotinylated lectins to pancreatic tissue sections; acinar cell glycoconjugates were localized in situ with peroxidase and fluorescent techniques and lebin-gold complexes. The lectin-binding properties of both fasting rabbit and rat pancreas revealed extensive and specific heterogeneity of the acinar cell population, Light and electron microscopy demonstrated highly heterogeneous labeling of the zymogen granule contents of specific acinar cells with the lectins Ulex europaeus agglutinin (UEA) and Erythrina cristagalli (EGA), which also showed perferential labeling of peri-insular acini, Other lectins also demonstrated heterogeneous binding to specific cellular regions. The striking acinar cell heterogeneity confirms earlier predictions, and may eventually prove to be the cellular basis for the secretion of different enzyme mixtures from heterogeneous sources within the pancreas.