TRANSPARENT TESTA 4-mediated flavonoids negatively affect embryonic fatty acid biosynthesis in Arabidopsis
TRANSPARENT TESTA 4-mediated flavonoids negatively affect embryonic fatty acid biosynthesis in Arabidopsis
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透明测试 4 介导的类黄酮对拟南芥胚胎脂肪酸生物合成产生负面影响
DOI：
10.1111/pce.13402
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Jiang Lixi
中科院分区：
文献类型：
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作者：
Xuan Lijie;Zhang Cuicui;Yan Tao;Wu Dezhi;Hussain Nazim;Li Zhilan;Chen Mingxun;Pan Jianwei;Jiang Lixi
黄酮类化合物参与植物的许多生理过程。透明种皮4（TT4）在类黄酮生物合成的第一步起作用，TT4功能的丧失导致类黄酮缺乏。据报道，黄酮类化合物缺乏是浅色油料种子中脂肪酸含量增加的主要原因，但关于种子黄酮类化合物与脂肪酸生物合成之间关系的细节尚不清楚。在这项工作中，我们采用了遗传策略结合生化和细胞学检测，以确定种子黄酮类化合物对脂肪酸的生物合成inArabidopsis thaliana的影响。我们发现TT4介导的类黄酮对胚胎脂肪酸的生物合成有负面影响。杂交实验表明，种子类黄酮的生物合成以及该过程对脂肪酸生物合成的影响以母系依赖的方式控制。TT4功能的丧失激活了种子胚中的糖酵解，从而增强了脂肪酸的生物合成，但并没有提高种子粘液的产量。此外，TT4功能的丧失降低了PIN ‐FORMED 4的表达，随后增加了胚中生长素的积累。药理学和遗传学上，生长素水平的提高增强了种子脂肪酸的生物合成。这些结果表明，黄酮类化合物通过调节WRINKLE 1和生长素的运输，通过碳源的重新分配来影响脂肪酸的合成。
Flavonoids are involved in many physiological processes in plants. TRANSPARENT TESTA 4 (TT4) acts at the first step of flavonoid biosynthesis, and the loss of TT4 function causes a lack of flavonoid. Flavonoid deficiency is reportedly the main cause of increased fatty acid content in pale‐coloured oilseeds, but details regarding the relationship between seed flavonoids and fatty acid biosynthesis are elusive. In this work, we applied a genetic strategy combined with biochemical and cytological assays to determine the effect of seed flavonoids on the biosynthesis of fatty acids inArabidopsis thaliana. We showed thatTT4‐mediated flavonoids negatively affect embryonic fatty acid biosynthesis. A crossing experiment indicated that seed flavonoid biosynthesis and the impact of this process on fatty acid biosynthesis were controlled in a maternal line‐dependent manner. Loss of TT4 function activated glycolysis in seed embryos, thereby enhancing fatty acid biosynthesis, but did not improve seed mucilage production. Moreover, loss of TT4 function reducedPIN‐FORMED 4expression and subsequently increased auxin accumulation in embryos. Pharmacologically and genetically elevated auxin levels enhanced seed fatty acid biosynthesis. These results indicated that flavonoids affect fatty acid biosynthesis by carbon source reallocation via regulation ofWRINKLE1and auxin transport.