GLUTAMINE-STIMULATED MODIFICATION AND DEGRADATION OF GLUTAMINE-SYNTHETASE IN HEPATOMA TISSUE-CULTURE CELLS
GLUTAMINE-STIMULATED MODIFICATION AND DEGRADATION OF GLUTAMINE-SYNTHETASE IN HEPATOMA TISSUE-CULTURE CELLS
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DOI：
10.1016/0092-8674(76)90190-2
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发表时间：
1976-01-01
期刊：
影响因子：
64.5
通讯作者：
KULKA, RG
中科院分区：
文献类型：
--
作者：
ARAD, G;FREIKOPF, A;KULKA, RG
Effects of glutamine on glutamine synthetase (GS) activity of hepatoma tissue culture (HTC) cells were studied with the aid of a specific goat antirat GS serum. Immunodiffusion and immunoelectrophoretic showed that rat liver GS and HTC cell GS are immunologically similar but not identical. Immunotitrations of HTC cell extracts demonstrated that in cells incubated in high concentrations (5 mM) of glutamine, a cross reacting form of GS with a decreased enzyme-specific activity accumulated. On prolonged incubation of cells of high glutamine, there was net degradation of GS to form immunologically inactive products. Radioimmunoprecipitation experiments showed that glutamine acts by accelerating the degradation of preformed GS.
