Mus81-Eme1 are essential components of a Holliday junction resolvase
Mus81-Eme1 are essential components of a Holliday junction resolvase
复制标题
DOI：
10.1016/s0092-8674(01)00536-0
复制
发表时间：
2001-11-16
期刊：
影响因子：
64.5
通讯作者：
Russell, P
中科院分区：
文献类型：
--
作者：
Boddy, MN;Gaillard, PHL;Russell, P
Mus 81是一种与ERCC 1-XPF核苷酸切除修复核酸内切酶的XPF亚基相关的裂殖酵母蛋白，是减数分裂所必需的，并且对于应对停滞的复制叉很重要。这些过程需要解析被称为霍利迪连接的X形DNA结构。我们报告说，Mus 81和相关的蛋白质Eme 1是一种内切核酸酶，解决霍利迪路口成线性双链体产品的组件。Mus 81和Eme 1在减数分裂重组的后期阶段是必需的。mus 81减数分裂缺陷通过细菌霍利迪连接消退酶的表达而被拯救。这些发现构成了强有力的证据，Mus 81和Emel 1是核霍利迪连接解离酶的亚基。
Mus81, a fission yeast protein related to the XPF subunit of ERCC1-XPF nucleotide excision repair endonuclease, is essential for meiosis and important for coping with stalled replication forks. These processes require resolution of X-shaped DNA structures known as Holliday junctions. We report that Mus81 and an associated protein Eme1 are components of an endonuclease that resolves Holliday junctions into linear duplex products. Mus81 and Eme1 are required during meiosis at a late step of meiotic recombination. The mus81 meiotic defect is rescued by expression of a bacterial Holliday junction resolvase. These findings constitute strong evidence that Mus81 and Emel1 are subunits of a nuclear Holliday junction resolvase.