YAP1 regulates PPARG and RXR alpha expression to affect the proliferation and differentiation of ovine preadipocyte
YAP1 regulates PPARG and RXR alpha expression to affect the proliferation and differentiation of ovine preadipocyte
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DOI：
10.1002/jcb.29265
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发表时间：
2019-07-11
影响因子：
4
通讯作者：
Wang, Feng
中科院分区：
文献类型：
--
作者：
Deng, Kaiping;Ren, Caifang;Wang, Feng
脂肪组织的发育受一系列发育信号通路的调控。Hippo通路是一种与脂肪形成密切相关的新型信号级联反应。Hippo信号通路中的大多数组分已被证实在前脂肪细胞的增殖和分化过程中发挥重要作用，而YAP 1在哺乳动物脂肪组织发育中的作用尚不清楚。因此，我们通过体外和体内实验研究了YAP 1在绵羊脂肪组织发育中的作用。我们观察到皮下脂肪组织中的脂肪细胞大小随发育而增加。YAP 1在绵羊前脂肪细胞体外分化过程中表达下降，而在脂肪组织发育过程中表达增加。YAP 1基因敲除可显著促进绵羊前脂肪细胞的脂质积累和抑制其增殖。此外，我们观察到，YAP 1缺陷显着上调过氧化物酶体增殖物激活受体γ（PPARG）和维甲酸X受体α（RXR α）的表达。相反，YAP 1的过度表达导致前脂肪细胞分化、脂滴形成和PPARG表达的抑制。总之，我们的研究结果表明，YAP 1通过改变PPARG和RXR α的表达来调节绵羊前脂肪细胞的增殖和分化。
Adipose tissue development is regulated by a serial of developmental signaling pathways. The Hippo pathway is a novel signaling cascade closely associated with adipogenesis. While most of Hippo pathway components had been verified that have a vital role in preadipocytes proliferation and differentiation, little is known about the function of Yes-associated protein 1 (YAP1) in mammalian adipose tissue development. Therefore, we investigated the role of YAP1 in ovine adipose tissue development by in vitro and in vivo experiments. We observed that the adipocyte size in subcutaneous adipose tissue increased with development. YAP1 expression increased during adipose tissue development, while decreased during the differentiation of ovine preadipocytes in vitro. YAP1 knockdown notably promoted lipid accumulation and suppressed ovine preadipocyte proliferation. In addition, we observed that YAP1 deficiency significantly upregulated peroxisome proliferator-activated receptor gamma (PPARG) and retinoid X receptor alpha (RXR alpha) expression. By contrast, overexpression of YAP1 led to the suppression of preadipocyte differentiation, lipid droplets formation, and PPARG expression. In brief, our findings demonstrated that YAP1 regulates the proliferation and differentiation of ovine preadipocyte via altering PPARG and RXR alpha expression.