A novel rat medial prefrontal cortical slice preparation to investigate synaptic transmission from amygdala to layer V prelimbic pyramidal neurons.
A novel rat medial prefrontal cortical slice preparation to investigate synaptic transmission from amygdala to layer V prelimbic pyramidal neurons.
复制标题
一种新型大鼠内侧前额叶皮质切片制剂，用于研究从杏仁核到 V 层前边缘锥体神经元的突触传递。
DOI：
10.1016/j.jneumeth.2005.07.002
复制
发表时间：
2006
影响因子：
3
通讯作者：
Gallagher,JoelP
中科院分区：
文献类型：
--
作者：
Orozco-Cabal,Luis;Pollandt,Sebastian;Liu,Jie;Vergara,Leoncio;Shinnick-Gallagher,Patricia;Gallagher,JoelP
体外制备的CNS切片中识别的突触的电生理记录为了解神经元回路的连通性以及负责神经元兴奋性和突触传递的潜在细胞机制提供了重要信息。我们提出了一种新的脑片制备(BLA-mPFC)的解剖学、电生理学和药理学特征，以研究基底外侧杏仁核突触对大鼠第V层内侧前额叶皮质锥体神经元的输入。用单侧注入杏仁核基底外侧核的荧光示踪剂(DII)，在前额叶脑片内检测支配前额叶和下缘皮质V层的杏仁核传出纤维。在体外，刺激急性BLA-mPFC切片内杏仁核基底外侧通路终末所引起的突触反应，通过细胞外和细胞内记录，在来自前皮质的V层锥体神经元中产生单突触兴奋性突触后反应。BLA-mPFC准备提供了杏仁核输入到内侧前额叶皮质的基本知识，在那里来自不同大脑区域的信息被整合并返回到皮质下结构，如杏仁核本身。除了研究正常的突触功能外，这项准备工作还提供了在长期接受药物治疗的动物身上研究这种突触的机会，或者已经通过基因操作来模拟已知涉及前额叶皮质和/或杏仁核病理的特定精神疾病(例如，精神分裂症、成瘾、焦虑和抑郁)。
Electrophysiological recordings from identified synapses in CNS slice preparations in vitro provide important information regarding the connectivity of neuronal circuits and the underlying cellular mechanisms responsible for neuronal excitability and synaptic transmission. We present an anatomical, electrophysiological, and pharmacological characterization of a novel brain slice preparation (BLA-mPFC) to investigate basolateral amygdala synaptic input to rat layer V medial prefrontal cortex pyramidal neurons. A fluorescent tracer (DiI) unilaterally infused in vivo into the basolateral amygdala was used to detect amygdala efferent fibers innervating layer V of the prelimbic and infralimbic cortices within prefrontal cortex slices. In vitro, evoked synaptic responses elicited by stimulating identified basolateral amygdala pathway terminals within the acute BLA-mPFC slice preparation yielded monosynaptic excitatory postsynaptic responses in layer V pyramidal neurons from the prelimbic cortex as determined by extracellular and intracellular recordings. The BLA-mPFC preparation provides essential knowledge of amygdaloid input to the medial prefrontal cortex where information from various brain areas is integrated and returned to subcortical structures, such as the amygdala itself. In addition to investigating normal synaptic function, this preparation provides opportunities to investigate this synapse in animals which have received drugs chronically or have been manipulated genetically to model specific mental diseases known to involve prefrontal cortex and/or amygdala pathology (e.g., schizophrenia, addiction, anxiety, and depression).