The effects of buffer condition on the fouling behavior of MVM virus filtration of an Fc‐fusion protein
The effects of buffer condition on the fouling behavior of MVM virus filtration of an Fc‐fusion protein
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缓冲液条件对 Fc 融合蛋白 MVM 病毒过滤污染行为的影响
DOI：
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发表时间：
2019
影响因子：
3.8
通讯作者：
X. Qian
中科院分区：
文献类型：
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作者：
Fnu Namila;Da Zhang;Steven J Traylor;Tung Nguyen;Nripen Singh;Ranil Wickramasinghe;X. Qian
将孔堵塞和滤饼过滤相结合的模型应用于Fc融合蛋白的病毒过滤，使用了三种商用过滤器F-1、F-2和F-3，在一系列缓冲条件下，包括磷酸钠缓冲液和三醋酸酯缓冲液，在pH值为7.5的200 mMNaC l和不含200 mMNaC l的情况下。该模型在所有溶液条件下均能很好地描述三种过滤器对小鼠微量病毒饲料溶液的污染行为。这表明病毒过滤器在过滤初期以孔堵塞机制为主，在过滤后期以滤饼过滤机制为主。该模型可以很好地描述膜通量和跨膜阻力。膜的阻塞率和膜上蛋白质层阻力的增加速率受膜的性质以及溶液条件的影响，这是由于溶液条件对病毒、蛋白质和膜之间的相互作用进行了调节。
A combined pore blockage and cake filtration model was applied to the virus filtration of an Fc‐fusion protein using the three commercially available filters, F‐1, F‐2, and F‐3 in a range of buffer conditions including sodium‐phosphate and tris‐acetate buffers with and without 200 mM NaCl at pH 7.5. The fouling behaviors of the three filters for the feed solutions spiked with minute virus of mice were described well by this combined model for all the solution conditions. This suggests that fouling of the virus filters is dominated by the pore blockage mechanism during the initial stage of the filtration and transformed to the cake filtration mechanism during the later stage of the filtration. Both flux and transmembrane resistance can be described well by this model. The pore blockage rate and the rate of increase of protein layer resistance over blocked pores are found to be affected by membrane properties as well as the solution conditions resulting from the modulation of interactions between virus, protein, and membrane by the solution conditions.