Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species
Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species
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DOI：
10.1016/j.biortech.2012.11.030
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发表时间：
2013-02-01
影响因子：
11.4
通讯作者：
Kawano, Shigeyuki
中科院分区：
文献类型：
--
作者：
Mizuno, Yusuke;Sato, Atsushi;Kawano, Shigeyuki
分析了缺硫对普通小球藻、小球藻、裂孔小球藻和克氏副小球藻4种小球藻生物量的影响。在缺硫条件下培养促进了淀粉的瞬时积累，然后是脂质储存的稳定增加。透射电子显微镜观察表明，在缺硫条件下，淀粉颗粒增加和减少，随后脂滴扩大。小球藻科在缺硫条件下积累的淀粉量是在硫充足条件下的1.5-2.7倍，积累的脂质量是在硫充足条件下的1.5-2.4倍。在小球藻中积累的脂肪酸中，75%以上的脂肪酸是由小球藻中积累的。在硫充足条件下培养的红毛酸浆中饱和脂肪酸含量为24.3%~ 59.7%，而在硫缺乏条件下培养的红毛酸浆中饱和脂肪酸含量为24.3%~ 59.7%。这些结果表明，淀粉和脂质的顺序积累是对硫消耗的反应，通常发生在小球藻属。(C)2012爱思唯尔有限公司保留所有权利。
The influence of sulfur deficiency on biomass production was analyzed in the four Chlorellaceae species, Chlorella vulgaris, Chlorella sorokiniana, Chlorella lobophora, and Parachlorella kessleri. Culturing under sulfur-deficient conditions promoted transient accumulation of starch followed by a steady increase in lipid storage. Transmission electron microscopy indicated an increase and decrease in starch granules and subsequent enlargement of lipid droplets under sulfur-deficient conditions. Chlorellaceae spp. accumulated 1.5-2.7-fold higher amounts of starch and 1.5-2.4-fold higher amounts of lipid under sulfur-deficient conditions than under sulfur-sufficient conditions. More than 75% of the fatty acids that accumulated in Chlorellaceae spp. under the sulfur-sufficient condition were unsaturated and culturing under sulfur-deficient conditions increased the saturated fatty acid content from 24.3% to 59.7% only in P. kessleri. These results indicate that the sequential accumulation of starch and lipid is a response to the sulfur depletion that commonly occurs in Chlorellaceae spp. (C) 2012 Elsevier Ltd. All rights reserved.