Alteration of the expression of the hypocretin (orexin) gene by 2-deoxyglucose in the rat lateral hypothalamic area
Alteration of the expression of the hypocretin (orexin) gene by 2-deoxyglucose in the rat lateral hypothalamic area
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DOI：
10.1097/00001756-200002280-00021
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发表时间：
2000-02-28
期刊：
影响因子：
1.7
通讯作者：
Griffond, B
中科院分区：
文献类型：
--
作者：
Bayer, L;Colard, C;Griffond, B
2-脱氧葡萄糖（2DG），已知诱导细胞内葡萄糖减少症的葡萄糖的非代谢类似物，腹膜内注射后，下丘脑泌素（Hcrt）/食欲素mRNA的水平进行性下降，观察到在大鼠外侧下丘脑，而黑色素浓集激素（MCH）在邻近神经元的表达保持不受影响。这一结果与先前报道的胰岛素刺激Hcrt表达一起证实了Hcrt神经元而非MCH神经元对葡萄糖可用性敏感，并表明它们通过不同的机制和/或不同的途径对细胞内葡萄糖减少症和低血糖状况作出反应。NeuroReport 11：531-533（C）2000 Lippincott威廉姆斯和威尔金斯。
Following an i.p. injection of 2-deoxyglucose (2DG), a nonmetabolizable analogue of glucose known to induce intracellular glucopenia, a progressive decrease in the level of hypocretin (Hcrt)/orexin mRNA was observed in the rat lateral hypothalamus while the melanin-concentrating hormone (MCH) expression in neighbouring neurons remained unaffected. This result together with the previously reported stimulation of Hcrt expression by insulin confirms that Hcrt neurons, but not MCH neurons, are sensitive to glucose availability and suggests that they respond through different mechanisms and/or different pathways to intracellular glucopenia and hypoglycemic conditions. NeuroReport 11:531-533 (C) 2000 Lippincott Williams & Wilkins.