Cultivated Single-Stranded DNA Phages That Infect Marine Bacteroidetes Prove Difficult To Detect with DNA-Binding Stains
Cultivated Single-Stranded DNA Phages That Infect Marine Bacteroidetes Prove Difficult To Detect with DNA-Binding Stains
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DOI：
10.1128/aem.06580-11
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发表时间：
2012-02-01
影响因子：
4.4
通讯作者：
Riemann, Lasse
中科院分区：
文献类型：
--
作者：
Holmfeldt, Karin;Odic, Dusko;Riemann, Lasse
这是第一次描述的栽培二十面体单链DNA（ssDNA）的异养海洋浮游细菌和Bacteroidetes主机上分离。8个样本中没有一个用DNA结合染色剂染色良好，这表明使用传统的计数方法大大低估了ssDNA的原位丰度。
This is the first description of cultivated icosahedral single-stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA-binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.