Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber
Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber
复制标题
DOI：
10.1016/j.envexpbot.2007.11.003
复制
发表时间：
2008-05
影响因子：
5.7
通讯作者：
Q. Shi;Zhujun Zhu
中科院分区：
文献类型：
--
作者：
Q. Shi;Zhujun Zhu
研究了水杨酸（SA）对黄瓜（Cucumis sativus L.）通过调查症状、植株生长、膜脂过氧化、抗氧化酶和抗氧化剂等指标，研究了大豆叶片的抗氧化特性。过量Mn会引起黄瓜植株严重黄化，抑制黄瓜生长，并显著增加黄瓜地上部和根系中Mn的积累，抑制Ca、Mg和Zn的吸收。SA的添加减少了Mn从根向地上部的运输，缓解了过量Mn对Ca、Mg、Zn吸收的抑制，减轻了毒害症状，促进了植株生长。过量Mn胁迫下，黄瓜叶片活性氧（ROS）的积累显著增加，导致膜脂过氧化，表现为硫代巴比妥酸反应物质（TBARS）的积累。SA的添加显著降低了ROS水平和脂质过氧化作用。抗氧化酶活性表现出不同程度的变化，SA处理抑制过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性，提高超氧化物歧化酶（SOD）、过氧化物酶（POD）、脱氢抗坏血酸还原酶（达尔）和谷胱甘肽还原酶（GR）活性。SA处理显著提高了高锰胁迫下黄瓜叶片中抗坏血酸和谷胱甘肽的含量。
The effects of salicylic acid (SA) on manganese (Mn) toxicity in cucumber plants (Cucumis sativus L.) were studied by investigating the symptoms, plant growth, lipid peroxidation, antioxidative enzymes and antioxidants. Excess Mn caused serious chlorosis and inhibited the growth of cucumber plants, and dramatically increased accumulation of Mn in both shoots and roots, furthermore, inhibited the absorption of Ca, Mg and Zn. Addition of SA decreased the transport of Mn from roots to shoots, alleviated the inhibition of Ca, Mg and Zn absorption induced by excess Mn, reduced the toxicity symptoms and promoted the plant growth. The accumulation of reactive oxygen species (ROS) significantly increased in cucumber leaves exposed to excess Mn, and resulted in the lipid peroxidation, which was indicated by accumulated concentration of thiobarbituric acid-reactive substances (TBARS). Addition of SA significantly decreased the level of ROS and lipid peroxidation. Activities of antioxidant enzymes showed different changes, addition of SA inhibited catalase (CAT) and ascorbate peroxidase (APX) activities, while increased activities of superoxide dismutase (SOD), peroxidase (POD), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) in cucumber leaves exposed to excess Mn. As important antioxidants, ascorbate and glutathione contents in cucumber leaves exposed to excess Mn were significantly increased by SA treatment.