Cytokeratin localization and basal cell differentiation in the epididymal epithelium during postnatal development of the mouse
Cytokeratin localization and basal cell differentiation in the epididymal epithelium during postnatal development of the mouse
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DOI：
10.2535/ofaj.91.83
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发表时间：
2015-02-01
期刊：
影响因子：
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通讯作者：
Yoshinaga, Kazuya
中科院分区：
文献类型：
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作者：
Yoshida, Ayaka;Murakami, Kana;Yoshinaga, Kazuya
附睾是男性生殖器官，具有多种功能，包括精子的浓缩、成熟和储存。附睾上皮由主细胞、透明细胞和基底细胞组成。为了全面了解基底细胞的发生和形态分化，我们研究了细胞角蛋白（CKs）在小鼠生后发育过程中附睾上皮的表达和定位。免疫组织化学染色显示，在成年小鼠，CK 5和CK 14只表达于基底细胞的胞浆中。在出生后的发育过程中，CK 5和CK 14染色阳性的基底细胞首先出现在1周龄小鼠的未成熟柱状上皮细胞中。免疫反应性在2-3周龄的小鼠中逐渐增强。在3周龄的小鼠中，IV和V区的免疫反应性较强。在>= 4周龄的小鼠中，在所有附睾区均观察到较强的免疫反应性。CK 5和CK 14可作为附睾基底细胞分化的标志物。这些基底细胞起源于未成熟的柱状上皮细胞，有圆顶形和瓶形两种类型。瓶状细胞主要位于小鼠附睾的起始段。
The epididymis is a male genital organ that has plays various functions, including sperm concentration, maturation, and storage. The epididymal epithelium consists of principal cells, clear cells, and basal cells. To comprehensively understand the occurrence and morphological differentiation of basal cells, we examined the expression and localization of cytokeratins (CKs) in the epididymal epithelium during postnatal development of the mouse. Immunohistochemical staining showed that, in adult mice, CK5 and CK14 were exclusively expressed in the cytoplasm of basal cells. During postnatal development, basal cells that stained positive for CK5 and CK14 first appeared in immature columnar epithelial cells in mice aged 1 week. The immunoreactivity became progressively stronger in mice aged 2-3 weeks. In mice aged 3 weeks, the immunoreactivity was strong in regions IV and V. In mice aged >= 4 weeks, strong immunoreactivity was observed in all epididymal regions. CK5 and CK14 could be useful markers of differentiation in epididymal basal cells. These basal cells originate from immature columnar epithelial cells and are of two types dome-shaped and flask-shaped. The flask-shaped cells are mainly located in the initial segment of the mouse epididymis.