Apple russeting as seen through the RNA-seq lens: strong alterations in the exocarp cell wall
Apple russeting as seen through the RNA-seq lens: strong alterations in the exocarp cell wall
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DOI：
10.1007/s11103-015-0303-4
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发表时间：
2015-05-01
影响因子：
5.1
通讯作者：
Hausman, Jean-Francois
中科院分区：
文献类型：
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作者：
Legay, Sylvain;Guerriero, Gea;Hausman, Jean-Francois
红果化是苹果（Malusxapartica）外果皮的一种重要缺陷，其主要特征是木栓质在外表皮细胞层细胞壁内部的积累。然而，关于触发这种特征的潜在遗传成分的知识仍然很粗略。对三个赤霉病苹果和三个糯苹果品种的外果皮进行了大量转录组学分析。选择该实验设计以降低基因型对所得结果的影响。对代表性基因和其他品种进行qPCR验证。基因本体富集揭示了抑制木质素和角质层的生物合成基因russeted外果皮，伴随着增强的木栓质沉积，应激反应，初级传感，NAC和MYB家族转录因子的表达，和特定的三萜生物合成基因。值得注意的是，MYB 93样转录因子的表达和木栓质生物合成的关键基因之间的强相关性（R-2 = 0.976）被发现。我们的研究结果表明，russeting诱导角质层生物合成基因的表达减少，导致应激反应，不仅影响木栓质沉积，而且还影响整个结构的细胞壁。本研究中鉴定的大量候选基因为进一步的功能研究提供了坚实的基础。
Russeting, a commercially important defect in the exocarp of apple (Malus x domestica), is mainly characterized by the accumulation of suberin on the inner part of the cell wall of the outer epidermal cell layers. However, knowledge on the underlying genetic components triggering this trait remains sketchy. Bulk transcriptomic profiling was performed on the exocarps of three russeted and three waxy apple varieties. This experimental design was chosen to lower the impact of genotype on the obtained results. Validation by qPCR was carried out on representative genes and additional varieties. Gene ontology enrichment revealed a repression of lignin and cuticle biosynthesis genes in russeted exocarps, concomitantly with an enhanced expression of suberin deposition, stress responsive, primary sensing, NAC and MYB-family transcription factors, and specific triterpene biosynthetic genes. Notably, a strong correlation (R-2 = 0.976) between the expression of a MYB93-like transcription factor and key suberin biosynthetic genes was found. Our results suggest that russeting is induced by a decreased expression of cuticle biosynthetic genes, leading to a stress response which not only affects suberin deposition, but also the entire structure of the cell wall. The large number of candidate genes identified in this study provides a solid foundation for further functional studies.