Effects of carbon-ion beams on human pancreatic cancer cell lines that differ in genetic status
Effects of carbon-ion beams on human pancreatic cancer cell lines that differ in genetic status
复制标题
DOI：
10.1097/01.coc.0000046037.75545.ad
复制
发表时间：
2004-02-01
影响因子：
2.6
通讯作者：
Ochiai, T
中科院分区：
文献类型：
--
作者：
Matsui, Y;Asano, T;Ochiai, T
在3个不同的线性能量转移（LET）值（13，50，和80千电子伏/妈妈）的重离子医用加速器在千叶对人类胰腺癌细胞系不同的遗传状态的碳离子束的相对生物有效性（RBE）进行了测定。RBE值计算为D10，相对于X射线，将存活分数降低至10%所需的剂量（戈伊）。应用形态学观察和流式细胞术分析细胞凋亡情况及D10与细胞周期检查点的关系。由D10值计算的RBE值范围为1.16至1.77的13千电子伏/妈妈束和1.83至2.46的80千电子伏/妈妈束。观察到各细胞系的D10值与G2/M期阻滞强度之间的相关性。与此相反，LET值并没有明确相关的诱导细胞凋亡。这些结果表明，碳离子束治疗是一种很有前途的方式。阐明G2/M期阻滞和细胞凋亡的机制可能为增强放射治疗胰腺癌的效应提供线索。
The relative biologic effectiveness (RBE) of carbon-ion beams at 3 different linear energy transfer (LET) values (13, 50, and 80 keV/mum) accelerated by the Heavy Ion Medical Accelerator in Chiba on human pancreatic cancer cell lines differing in genetic status was determined. The RBE values were calculated as D10, the dose (Gy) required to reduce the surviving fraction to 10%, relative to X-rays. We also investigated apoptosis and the relationship between D10 and the cell cycle checkpoint using morphologic examination and flow cytometry analysis, respectively. The RBE values calculated by the D10 values ranged from 1.16 to 1.77 for the 13-keV/mum beam and from 1.83 to 2.46 for the 80-keV/mum beam. A correlation between the D10 values of each cell line and intensity of G2/M arrest was observed. In contrast, LET values did not clearly correlate with induction of apoptosis. These results suggest that carbon-ion beam therapy is a promising modality. Elucidation of the mechanisms of G2/M arrest and apoptosis may provide clues to enhancing the effects of radiation on pancreatic cancer.