Optical coherence tomography and histologic measurements of nerve fiber layer thickness in normal and glaucomatous monkey eyes
Optical coherence tomography and histologic measurements of nerve fiber layer thickness in normal and glaucomatous monkey eyes
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DOI：
10.1167/iovs.06-0876
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发表时间：
2007-08-01
影响因子：
4.4
通讯作者：
Wollstein, Gadi
中科院分区：
文献类型：
--
作者：
Schuman, Joel S.;Pedut-Kloizman, Tamar;Wollstein, Gadi
目的.目的：探讨视网膜神经纤维层（RNFL）厚度变化的光学相干断层扫描（OCT）测量方法，并将OCT RNFL厚度测量结果与相同组织的形态学测量结果进行比较。用氩激光对12只食蟹猴的每只眼睛的前房角进行治疗以诱导IOP升高。在激光损伤后14周，每周对每只猴的治疗眼和对侧眼进行OCT测量。实验结束时杀死猴子，并对同一只眼睛的终末OCT RNFL测量值和定量组织形态测量评估进行比较。暴露于升高的IOP对RNFL的影响通过混合效应模型表征。线性混合效应模型提供了平衡和不平衡重复测量数据的无偏分析，检测组效应（固定效应）和个体受试者效应（随机效应），从而最大限度地利用所有可用数据。10眼眼压升高。暴露于高眼压与平均RNFL厚度的损失相关，平均损失率为3.77 +/- 0.08 μ m/周。平均而言，OCT RNFL厚度测量值比组织形态学测量值高5.7 μ m（95%置信区间，3.6-7.8; P = 0.003）。在一组实验性青光眼猴眼中，OCT证实了与IOP升高相关的RNFL变薄。OCT测量结果与相同组织的组织形态测量结果一致。
PURPOSE. To investigate optical coherence tomography (OCT) measurements of retinal nerve fiber layer (RNFL) thickness change associated with elevated intraocular pressure (IOP) over a period of time, and to compare in vivo OCT RNFL thickness measurements with morphologic measurements of the same tissues.METHODS. One eye of each of 12 cynomolgus monkeys was treated with argon laser to the anterior chamber angle to induce elevated IOP. OCT measurements were made weekly in the treated and the contralateral eyes of each monkey for 14 weeks after the laser insult. The monkeys were killed at the conclusion of the experiment, and comparisons were made between the terminal OCT RNFL measurements and quantitative histomorphometric assessments in the same eyes. Effects of exposure to elevated IOP on RNFL were characterized by a mixed-effects model. Linear mixed-effects models provided unbiased analysis of balanced and unbalanced repeated-measures data, detection of group effects (fixed effects), and individual subject effects (random effects), thereby making the best use of all available data.RESULTS. Increased IOP was achieved in 10 eyes. Exposure to high IOP was associated with the loss of mean RNFL thickness at a median rate of 3.77 +/- 0.08 mu m/wk. On average, OCT RNFL thickness measurements were higher than histomorphologic measurements by 5.7 mu m (95% confidence interval, 3.6-7.8; P = 0.003).CONCLUSIONS. Thinning of the RNFL associated with elevated IOP was demonstrated with OCT in a group of experimentally glaucomatous monkey eyes over a period. OCT measurements corresponded with histomorphometric measurements of the same tissues.