Recombinational DSBs-intersected genes converge on specific disease- and adaptability-related pathways
Recombinational DSBs-intersected genes converge on specific disease- and adaptability-related pathways
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重组 DSB 交叉基因汇聚于特定疾病和适应性相关途径
DOI：
10.1093/bioinformatics/bty376
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发表时间：
2018
期刊：
影响因子：
5.8
通讯作者：
Gao Feng
中科院分区：
文献类型：
--
作者：
Yang Zhi Kai;Luo Hao;Zhang Yanming;Wang Baijing;Gao Feng
芽殖酵母是研究真核生物重组的一种模式生物。虽然已经通过实验方法对该物种进行了许多重组研究，但迫切需要基于生物信息学分析的群体基因组研究，以大大提高重组检测的范围和准确性。在这里，我们进行了群体基因组分析的重组在S.党aeto揭示重组和真核生物的进化之间的潜在rules.Resultsby群体基因组分析，我们发现显着更多和更长的重组事件在临床菌株，这表明，不利的环境条件下创建了一个明显更广泛的遗传组合，以应对选择压力。基于对重组双链断裂（DSB）-修复基因（RDIGs）的分析，我们发现RDIGs显著地集中于特定的疾病和适应性相关通路，表明重组在与疾病和环境适应性相关的DSB修复中起着生物学上的关键作用，特别是在人类神经系统疾病中。通过对RDIGs的进化分析，我们发现在酵母群体中高度流行的RDIGs往往在进化上更加保守，这表明这些RDIGs中DSB的准确修复对于确保真核生物的生存或适应性至关重要。
MotivationThe budding yeastSaccharomyces cerevisiaeis a model species powerful for studying the recombination of eukaryotes. Although many recombination studies have been performed for this species by experimental methods, the population genomic study based on bioinformatics analyses is urgently needed to greatly increase the range and accuracy of recombination detection. Here, we carry out the population genomic analysis of recombination inS.cerevisiaeto reveal the potential rules between recombination and evolution in eukaryotes.ResultsBy population genomic analysis, we discover significantly more and longer recombination events in clinical strains, which indicates that adverse environmental conditions create an obviously wider range of genetic combination in response to the selective pressure. Based on the analysis of recombinational double strand breaks (DSBs)-intersected genes (RDIGs), we find that RDIGs significantly converge on specific disease- and adaptability-related pathways, indicating that recombination plays a biologically key role in the repair of DSBs related to diseases and environmental adaptability, especially the human neurological disorders. By evolutionary analysis of RDIGs, we find that the RDIGs highly prevailing in populations of yeast tend to be more evolutionarily conserved, indicating the accurate repair of DSBs in these RDIGs is critical to ensure the eukaryotic survival or fitness.Supplementary informationSupplementary data are available atBioinformaticsonline.