Repeated evolution of circadian clock dysregulation in cavefish populations
Repeated evolution of circadian clock dysregulation in cavefish populations
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DOI：
10.1101/2020.01.14.906628
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发表时间：
2020-01
期刊：
影响因子：
4.5
通讯作者：
Katya L. Mack;James B. Jaggard;J. Persons;Emma Y. Roback;C. Passow;Bethany A. Stanhope;Estephany Ferrufino;Dai Tsuchiya;Sarah E Smith;Brian D. Slaughter;J. Kowalko;Nicolas Rohner;A. Keene;S. McGaugh
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文献类型：
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作者：
Katya L. Mack;James B. Jaggard;J. Persons;Emma Y. Roback;C. Passow;Bethany A. Stanhope;Estephany Ferrufino;Dai Tsuchiya;Sarah E Smith;Brian D. Slaughter;J. Kowalko;Nicolas Rohner;A. Keene;S. McGaugh
昼夜节律在自然界中几乎无处不在，这表明它们对于在不同环境中生存至关重要。居住在昼夜节律不齐的环境中的生物，例如洞穴，为研究昼夜节律的演变以应对不断变化的生态压力提供了独特的机会。在这里，我们证明洞穴环境导致墨西哥洞穴鱼多个种群的生物钟反复被破坏，昼夜节律转录组显示节律性广泛降低以及核心起搏器基因激活/抑制时间的变化。然后，我们研究了穴居鱼中节律性表达降低的两个基因的功能。这些基因的突变体在多个洞穴种群中观察到睡眠表型减少，这表明昼夜节律失调与睡眠减少之间存在联系。总而言之，我们的结果表明，心律失常环境中的进化通过不同的分子机制导致多个人群的生物钟失调。
Circadian rhythms are nearly ubiquitous throughout nature, suggesting they are critical for survival in diverse environments. Organisms inhabiting environments with arrhythmic days, such as caves, offer a unique opportunity to study the evolution of circadian rhythms in response to changing ecological pressures. Here we demonstrate that the cave environment has led to the repeated disruption of the biological clock across multiple populations of Mexican cavefish, with the circadian transcriptome showing widespread reductions in rhythmicity and changes to the timing of the activation/repression of genes in the core pacemaker. Then, we investigate the function of two genes with decreased rhythmic expression in cavefish. Mutants of these genes phenocopy reductions in sleep seen in multiple cave populations, suggesting a link between circadian dysregulation and sleep reduction. Altogether, our results reveal that evolution in an arrhythmic environment has resulted in dysregulation to the biological clock across multiple populations by diverse molecular mechanisms.