Relevance of HTLV-1 proviral load in asymptomatic and symptomatic patients living in endemic and non-endemic areas of Argentina
Relevance of HTLV-1 proviral load in asymptomatic and symptomatic patients living in endemic and non-endemic areas of Argentina
复制标题
居住在阿根廷流行区和非流行区的无症状和有症状患者的 HTLV-1 前病毒载量的相关性
作者：
M. Pineda;M. B. Bouzas;M. Remesar;Ariel Fridman;C. Remondegui;L. Mammana;Natalia Altamirano;P. Paradiso;P. Costantini;Luciana Tadey;P. Aulicino;A. Mangano
外周血单核细胞 (PBMC) 中的 HTLV-1 前病毒载量 (pVL) 被提议作为疾病进展的标志物，但其作用仍然存在争议。本研究的目的是评估有症状患者和无症状 HTLV-1 携带者的 HTLV-1 pVL 水平。在这项横断面研究中，通过实时 PCR 测量了 102 名无症状携带者和 22 名有症状患者（5 名 ATLL、15 名 TSP 和 2 名葡萄膜炎患者）的 pVL。我们观察到，与无症状 HTLV-1 携带者（中位数 = 4.38 log10 HTLV-1 拷贝/106 PBMC；p = 0.0030）相比，有症状患者的 HTLV-1 pVL 显着较高（中位数 = 4.99 log10 HTLV-1 拷贝/106 PBMC）。在无症状 HTLV-1 携带者中观察到 HTLV-1 pVL 水平存在很大差异，其中一些 pVL 与有症状患者中观察到的一样高。根据出生地对无症状HTLV-1携带者进行分类，来自阿根廷北部流行区的患者中检测到pVL水平最高。我们的结果强化了前病毒载量作为 HTLV-1 疾病进展的预后标志物的有用性。此外，不能排除宿主、病毒或社会环境因素作为高原病毒载量的决定因素。
HTLV-1 proviral load (pVL) in peripheral blood mononuclear cell (PBMCs) is proposed as a marker of disease progression but its role still remains controversial. The aim of this study was to evaluate the levels of HTLV-1 pVL in symptomatic patients and asymptomatic HTLV-1 carriers. In this cross-sectional study the pVL was measured by Real Time PCR in 102 asymptomatic carriers and 22 symptomatic patients (5ATLL, 15 TSP and 2 uveitis). We observed that the HTLV-1 pVL was significantly higher in symptomatic patients (median = 4.99 log10 HTLV-1 copies /106 PBMCs) compared to asymptomatic HTLV-1 carriers (median = 4.38 log10 HTLV-1 copies /106 PBMCs; p = 0.0030). A wide variation on the HTLV-1 pVL levels among asymptomatic HTLV-1 carriers was observed with some pVL as high as those observed in symptomatic patients. The asymptomatic HTLV-1 carriers were divided according to the place of birth and the highest levels of pVL were detected among patients from endemics areas from the North of Argentina. Our results reinforce the usefulness of the proviral load would be a prognostic marker of HTLV-1 disease progression. Moreover, host, viral or socio-environmental factors cannot be excluded as determinant of high proviral load.