Genomic variation in the hybrids of white crucian carp and red crucian carp: evidence from ribosomal DNA
Genomic variation in the hybrids of white crucian carp and red crucian carp: evidence from ribosomal DNA
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白鲫鱼和红鲫鱼杂交种的基因组变异：来自核糖体DNA的证据
DOI：
10.1007/s11427-015-4835-2
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发表时间：
2015-06-01
影响因子：
9.1
通讯作者：
Liu ShaoJun
中科院分区：
文献类型：
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作者：
Wang Jing;Xiao Jun;Liu ShaoJun
在本研究中，我们进行了白鲤(雌性)与红鲤(雄性)(WR)的杂交，并对其后代的形态、繁殖和遗传学进行了研究。与双亲不同，灰色的WR表现出双亲的杂交形态特征。WR具有正常的性腺，能产生成熟的卵子或精子，受精率高(90.2%)，孵化率高(80.5%)，有助于生产和扩大种群。WR与亲本DNA含量相同，为二倍体FISH，染色体数为100条(2n=100)。扩增出45S rDNA的ITS序列，其中WR的ITS-1区、S区和ITS-2区全长884bp。WR与其亲本的序列具有较高的相似性，并倾向于雄性遗传。在WR中，5S rDNA的核苷酸序列由三种长度类型组成，共654个碱基。序列分析表明，WR与母本的相似性为94.2%，与父本的相似性为95.1%。ITS和NTS的序列分析表明，杂交后代中存在重组和变异，这是适应和物种形成的遗传基础。综上所述，我们通过杂交获得了WR，它在形态和遗传组成上表现出与双亲本质不同的杂交性状。WR的获得对鱼类遗传育种具有重要意义。
In this study, we conducted a cross of white crucian carp (female) xred crucian carp (male) (WR), and characterized the morphology, reproduction and genetics of the progeny. Different from parents, WR with the gray color showed the hybrid morphological traits of both parents. WR possessed normal gonads producing mature eggs or sperm, and exhibited high fertilization rate (90.2%) and high hatchery rate (80.5%), which contributed to produce and enlarge the population. WR with the same DNA content as parents was a diploid fish with 100 chromosomes (2n= 100). Amplified ITS of 45S rDNA, in WR the sequences consisting of 884 bp bases of the entire ITS-1 region, 5.8 S region, and entire ITS-2 region. The sequences showed high similarity between WR and its parents and leaned towards male inheritance. In WR, NTS of 5S rDNA consisted of three length types with total 654 bp bases. From sequence analysis of NTS, WR shared 94.2% and 95.1% similarities with their female and male parent, respectively. Sequence analysis of ITS and NTS revealed that there existed recombination and variation in the hybrid progeny, which was the genetic base for adaptation and speciation. In conclusion, we obtained WR from hybridization and it exhibited hybrid traits in morphology and variation in genetic composition showing essential difference with its parents. The obtainment of WR has important significance in fish genetic breeding.