Arabidopsis flowering induced by photoperiod under 3-D clinostat rotational simulated microgravity
Arabidopsis flowering induced by photoperiod under 3-D clinostat rotational simulated microgravity
复制标题
3-D 回转器旋转模拟微重力下光周期诱导拟南芥开花
DOI：
10.1016/j.actaastro.2018.11.014
复制
发表时间：
2020
影响因子：
3.5
通讯作者：
Huiqiong Zheng
中科院分区：
文献类型：
--
作者：
Junyan Xie;Huiqiong Zheng
从营养生长到开花的过渡对于植物的繁殖成功是非常重要的。这一过程在太空微重力条件下会延迟，但微重力是否会在分子水平上影响植物开花仍是未知数。在这里，我们研究了改变重力对光周期控制开花诱导的影响，通过使用随机定位机（RPM，3-D回转器）。首先，在Col-0背景下使用转基因植物proFT：GFP并检查光周期途径中的关键基因如FT、CONSTANS（CO）和GIGANTEA（GI）的表达，我们发现在长日照条件下，3-D回转器旋转影响FLOWLOCUS T（FT）启动子的活性。其次，使用proSUC 2：FTCDS：GFP构建体在盗窃-10突变体的背景下，我们观察到FT从叶片到茎顶端分生组织的运输也被延迟模拟微重力下的3-D clinostation条件下相比，1 g固定对照。这些结果表明，光周期控制的植物开花延迟3-D回转器旋转。
The transition from vegetative growth to flowering is very important for the plant reproductive success. This process is delayed under microgravity conditions in space, but whether microgravity affects plant flowering at the molecular level is still unknown. Here, we studied the effects of altered gravity on photoperiod-controlled flowering induction by using a random positioning machine (RPM, 3-D clinostat). First, using transgenic plant proFT:GFPin the Col-0 background and examining the expression of key genes in the photoperiod pathway, such asFT,CONSTANS(CO) andGIGANTEA(GI), we found that 3-D clinostat rotation affected the activity of theFLOWERING LOCUS T(FT) promoter under long-day conditions. Second, using a proSUC2:FTCDS:GFPconstruct in theft-10mutant background, we observed that the transport of FT from the leaves to the shoot apical meristem was also delayed by simulated microgravity under 3-D clinostation conditions compared with the 1 g stationary control. These results indicate that photoperiod-controlled plant flowering is delayed by 3-D clinostat rotation.